Daily Intraparaventricular Orexin-A Treatment Induces Weight Loss in Rats

Overview

Journal Obesity (Silver Spring)

Specialties Endocrinology
Nutritional Sciences
Physiology

Date 2009 Apr 4

PMID 19343016

Citations 28

Authors

Colleen M Novak

James A Levine

Affiliations

Soon will be listed here.

Abstract

The neuropeptide orexin (hypocretin) increases energy expenditure partially through increasing spontaneous physical activity. The ability of exogenous orexin to alter body weight has never been established, however. We sought to determine whether orexin-A microinjected into the paraventricular nucleus of the hypothalamus (PVN) induced weight loss in rats. Chronic guide cannulae were implanted into rats, aimed at the PVN. Rats were given daily microinjections of orexin (0.5 nmol) or vehicle into the PVN for 6 days; food intake and body weight were measured daily. In a separate group of rats, we injected orexin-A and vehicle intra-PVN and measured daily activity levels. Daily orexin treatment induced weight loss: orexin-A-treated rats lost significantly more weight than their vehicle-injected counterparts without a significant difference in food intake. Rats were significantly more active after intra-PVN orexin compared to vehicle. These results support the concept that orexinergic agents have the potential to produce negative energy balance through increasing physical activity. This presents a promising, untapped potential resource for weight loss.

Citing Articles

Interactions between Lateral Hypothalamic Orexin and Dorsal Raphe Circuitry in Energy Balance.

Mavanji V, Pomonis B, Shekels L, Kotz C Brain Sci. 2024; 14(5).

PMID: 38790443 PMC: 11117928. DOI: 10.3390/brainsci14050464.

Physiological Role of Orexinergic System for Health.

Villano I, La Marra M, Di Maio G, Monda V, Chieffi S, Guatteo E Int J Environ Res Public Health. 2022; 19(14).

PMID: 35886210 PMC: 9323672. DOI: 10.3390/ijerph19148353.

Orexin, serotonin, and energy balance.

Mavanji V, Pomonis B, Kotz C WIREs Mech Dis. 2022; 14(1):e1536.

PMID: 35023323 PMC: 9286346. DOI: 10.1002/wsbm.1536.

Synchronous neuronal interactions in rat hypothalamic culture: a novel model for the study of network dynamics in metabolic disorders.

Mavanji V, Georgopoulos A, Kotz C Exp Brain Res. 2021; 239(3):755-764.

PMID: 33388905 DOI: 10.1007/s00221-020-05977-7.

Role of orexin-A in the ventrolateral preoptic area on components of total energy expenditure.

Coborn J, DePorter D, Mavanji V, Sinton C, Kotz C, Billington C Int J Obes (Lond). 2017; 41(8):1256-1262.

PMID: 28392556 PMC: 6893852. DOI: 10.1038/ijo.2017.92.

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