Differential Effects of Adrenocorticotropic Hormone on Human and Mouse Adipose Tissue

Overview

Journal J Comp Physiol B

Specialties Biochemistry
Endocrinology
Physiology

Date 2003 Aug 20

PMID 12925881

Citations 11

Authors

K Kiwaki

J A Levine

Affiliations

Soon will be listed here.

Abstract

Adrenocorticotropic hormone (ACTH) induces lipolysis in a dose-dependent fashion in rodent adipose tissue and adipocytes in vitro. The role of ACTH on lipolysis in human adipose tissue is less clear, however. In this study, we address the hypothesis that ACTH induces lipolysis in human adipose tissue. We used ex vivo organ culture to examine lipolysis in human and mouse adipose tissue. Adipose tissue fragments suspended in culture medium and human ACTH, isoproterenol (positive control), or insulin (negative control) was added in varying concentrations. Lipolysis was measured using glycerol appearance. ACTH receptor mRNA expression was assessed using reverse-transcription polymerase chain reaction (RT-PCR). In mouse adipose tissue, ACTH induced lipolysis in dose-dependent manner; 100 pmol/l ACTH induced 67+/-19% of isoproterenol-stimulated lipolysis and 500 pmol/l ACTH: 86+/-13%. In contrast, human adipose tissue shared no significant response to 100 pmol/l ACTH; ACTH was associated with 9+/-6% and 500 pmol/l of ACTH, 8+/-6% of isoproterenol-stimulated lipolysis. ACTH receptor mRNA was present in mouse adipose tissue, but undetectable in human adipose tissue. These results suggest lipolysis regulation differs between human and mouse adipose tissue in response to ACTH.

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Brown Adipose Tissue-A Translational Perspective.

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Non-Canonical Effects of ACTH: Insights Into Adrenal Insufficiency.

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Characterization of immortalized human brown and white pre-adipocyte cell models from a single donor.

Markussen L, Isidor M, Breining P, Andersen E, Rasmussen N, Petersen L PLoS One. 2017; 12(9):e0185624.

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References

Honnor R, Dhillon G, Londos C . cAMP-dependent protein kinase and lipolysis in rat adipocytes. II. Definition of steady-state relationship with lipolytic and antilipolytic modulators. J Biol Chem. 1985; 260(28):15130-8. View

Bousquet-Melou A, Galitzky J, Lafontan M, Berlan M . Control of lipolysis in intra-abdominal fat cells of nonhuman primates: comparison with humans. J Lipid Res. 1995; 36(3):451-61. View

Mountjoy K, Robbins L, Mortrud M, Cone R . The cloning of a family of genes that encode the melanocortin receptors. Science. 1992; 257(5074):1248-51. DOI: 10.1126/science.1325670. View

Ramachandran J, Lee V . Divergent effects of adrenocorticotropin and melanotropin on isolated rat and rabbit adipocytes. Biochim Biophys Acta. 1976; 428(2):339-46. DOI: 10.1016/0304-4165(76)90041-6. View

Boston B . The role of melanocortins in adipocyte function. Ann N Y Acad Sci. 2000; 885:75-84. DOI: 10.1111/j.1749-6632.1999.tb08666.x. View

Fried S, Moustaid-Moussa N . Culture of adipose tissue and isolated adipocytes. Methods Mol Biol. 2001; 155:197-212. DOI: 10.1385/1-59259-231-7:197. View

Londos C, Honnor R, Dhillon G . cAMP-dependent protein kinase and lipolysis in rat adipocytes. III. Multiple modes of insulin regulation of lipolysis and regulation of insulin responses by adenylate cyclase regulators. J Biol Chem. 1985; 260(28):15139-45. View

Saulnier-Blache J, Dandine M, Dauzats M, Daviaud D, Langin D . Increase in uncoupling protein-2 mRNA expression by BRL49653 and bromopalmitate in human adipocytes. Biochem Biophys Res Commun. 1999; 256(1):138-41. DOI: 10.1006/bbrc.1999.0303. View

Boston B, Cone R . Characterization of melanocortin receptor subtype expression in murine adipose tissues and in the 3T3-L1 cell line. Endocrinology. 1996; 137(5):2043-50. DOI: 10.1210/endo.137.5.8612546. View

10.

Fried S, Zechner R . Cachectin/tumor necrosis factor decreases human adipose tissue lipoprotein lipase mRNA levels, synthesis, and activity. J Lipid Res. 1989; 30(12):1917-23. View

11.

Slavin B, Elias J . The influence of pituitary hormones and norepinephrine on the size of adipose cells in organ culture. Anat Rec. 1969; 164(2):141-51. DOI: 10.1002/ar.1091640202. View

12.

Humphreys S, Fisher R, Frayn K . Micro-method for measurement of sub-nanomole amounts of triacylglycerol. Ann Clin Biochem. 1990; 27 ( Pt 6):597-8. DOI: 10.1177/000456329002700613. View

13.

Levine J, Jensen M, Eberhardt N, OBrien T . Adipocyte macrophage colony-stimulating factor is a mediator of adipose tissue growth. J Clin Invest. 1998; 101(8):1557-64. PMC: 508735. DOI: 10.1172/JCI2293. View

14.

Ramachandran J, Farmer S, Liles S, Li C . Comparison of the steroidogenic and melanotropic activities of corticotropin, alpha-melanotropin and analogs with their lipolytic activities in rat and rabbit adipocytes. Biochim Biophys Acta. 1976; 428(2):347-54. DOI: 10.1016/0304-4165(76)90042-8. View

15.

Rees L, Cook D, KENDALL J, Allen C, KRAMER R, Ratcliffe J . A radioimmunoassay for rat plasma ACTH. Endocrinology. 1971; 89(1):254-61. DOI: 10.1210/endo-89-1-254. View

16.

Honnor R, Dhillon G, Londos C . cAMP-dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation, and predictability in behavior. J Biol Chem. 1985; 260(28):15122-9. View

17.

Xue B, Wilkison W, Zemel M . The agouti gene product inhibits lipolysis in human adipocytes via a Ca2+-dependent mechanism. FASEB J. 1998; 12(13):1391-6. View

18.

Morimoto C, Tsujita T, Okuda H . Antilipolytic actions of insulin on basal and hormone-induced lipolysis in rat adipocytes. J Lipid Res. 1998; 39(5):957-62. View