Local ATP Generation by Brain-type Creatine Kinase (CK-B) Facilitates Cell Motility

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2009 Apr 1

PMID 19333390

Citations 24

Authors

Jan W P Kuiper

Remco van Horssen

Frank Oerlemans

Wilma Peters

Michiel M T van Dommelen

Mariska M te Lindert

Timo L M Ten Hagen

Edwin Janssen

Jack A M Fransen

Be Wieringa

Affiliations

Soon will be listed here.

Abstract

Background: Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocytotic activity of macrophages, processes sharing dependency on actomyosin dynamics.

Methodology/principal Findings: Here, we provide evidence for direct coupling between CK-B and actomyosin activities in cortical microdomains of astrocytes and fibroblasts during spreading and migration. CK-B transiently accumulates in membrane ruffles and ablation of CK-B activity affects spreading and migration performance. Complementation experiments in CK-B-deficient fibroblasts, using new strategies to force protein relocalization from cytosol to cortical sites at membranes, confirmed the contribution of compartmentalized CK-B to cell morphogenetic dynamics.

Conclusion/significance: Our results provide evidence that local cytoskeletal dynamics during cell motility is coupled to on-site availability of ATP generated by CK-B.

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