Microfluidic System for Formation of PC-3 Prostate Cancer Co-culture Spheroids

Overview

Journal Biomaterials

Specialty Biomedical Engineering

Date 2009 Mar 24

PMID 19304321

Citations 106

Authors

Amy Y Hsiao

Yu-Suke Torisawa

Yi-Chung Tung

Sudha Sud

Russell S Taichman

Kenneth J Pienta

Shuichi Takayama

Affiliations

Soon will be listed here.

Abstract

The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.

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