Selective Stabilization of Mammalian MicroRNAs by 3' Adenylation Mediated by the Cytoplasmic Poly(A) Polymerase GLD-2

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2009 Feb 26

PMID 19240131

Citations 242

Authors

Takayuki Katoh

Yuriko Sakaguchi

Kenjyo Miyauchi

Takeo Suzuki

Shin-Ichi Kashiwabara

Tadashi Baba

Tsutomu Suzuki

Affiliations

Soon will be listed here.

Abstract

The steady-state levels of microRNAs (miRNAs) and their activities are regulated by the post-transcriptional processes. It is known that 3' ends of several miRNAs undergo post-dicing adenylation or uridylation. We isolated the liver-specific miR-122 from human hepatocytes and mouse livers. Direct analysis by mass spectrometry revealed that one variant of miR-122 has a 3'-terminal adenosine that is introduced after processing by Dicer. We identified GLD-2, which is a regulatory cytoplasmic poly(A) polymerase, as responsible for the 3'-terminal adenylation of miR-122 after unwinding of the miR-122/miR-122* duplex. In livers from GLD-2-null mice, the steady-state level of the mature form of miR-122 was specifically lower than in heterozygous mice, whereas no reduction of pre-miR-122 was observed, demonstrating that 3'-terminal adenylation by GLD-2 is required for the selective stabilization of miR-122 in the liver.

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