Differential NF-kappaB Pathways Induction by Lactobacillus Plantarum in the Duodenum of Healthy Humans Correlating with Immune Tolerance

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Feb 5

PMID 19190178

Citations 171

Authors

Peter van Baarlen

Freddy J Troost

Saskia van Hemert

Cindy van der Meer

Willem M de Vos

Philip J de Groot

Guido J E J Hooiveld

Robert-Jan M Brummer

Michiel Kleerebezem

Affiliations

Soon will be listed here.

Abstract

How do we acquire immune tolerance against food microorganisms and commensal bacteria that constitute the intestinal microbiota? We investigated this by stimulating the immune system of adults with commensal Lactobacillus plantarum bacteria. We studied the in vivo human responses to L. plantarum in a randomized double-blind placebo-controlled cross-over study. Healthy adults ingested preparations of living and heat-killed L. plantarum bacteria. Biopsies were taken from the intestinal duodenal mucosa and altered expression profiles were analyzed using whole-genome microarrays and by biological pathway reconstructions. Expression profiles of human mucosa displayed striking differences in modulation of NF-kappaB-dependent pathways, notably after consumption of living L. plantarum bacteria in different growth phases. Our in vivo study identified mucosal gene expression patterns and cellular pathways that correlated with the establishment of immune tolerance in healthy adults.

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