Improvement of Embryonic Development and Production of Offspring by Transferring Meiosis-II Chromosomes of Senescent Mouse Oocytes into Cytoplasts of Young Mouse Oocytes

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2008 Dec 20

PMID 19096925

Citations 9

Authors

Akinori Mitsui

Midori Yoshizawa

Hiromichi Matsumoto

Emiko Fukui

Affiliations

Soon will be listed here.

Abstract

Purpose: The effects of reciprocal transplantation of meiosis-II chromosomes between senescent and young mouse oocytes were evaluated based on pre- and post-implantation development ability of resultant embryos.

Methods: Karyoplasts including meiosis-II chromosomes of oocytes from senescent Rockefeller mouse/Ms-Rb(6, 15) females (10 to 12 months, age-related infertile mice) were transferred into cytoplasts of oocytes from young F(1) females (3 to 5 months). Reconstructed oocytes were fertilized in vitro, and then the resultant embryos were cultured in vitro and transferred to recipient mice.

Results: The reconstructed oocytes that consisted of aged-karyoplasts and young-cytoplasts showed significantly improved embryonic development (from 23.2% to 30.0%) and development to term (from 6.3% to 27.1%, P < 0.05) as compared with the oocytes reconstructed from young-karyoplasts and aged-cytoplasts.

Conclusions: The present study showed successful rejuvenation for age-related infertility using transplantation of meiosis-II chromosomes in animal experimental models.

Citing Articles

Prospects of Germline Nuclear Transfer in Women With Diminished Ovarian Reserve.

Christodoulaki A, Boel A, Tang M, De Roo C, Stoop D, Heindryckx B Front Endocrinol (Lausanne). 2021; 12:635370.

PMID: 33692760 PMC: 7937897. DOI: 10.3389/fendo.2021.635370.

Mitochondrial Genetic Drift after Nuclear Transfer in Oocytes.

Yamada M, Akashi K, Ooka R, Miyado K, Akutsu H Int J Mol Sci. 2020; 21(16).

PMID: 32824295 PMC: 7461576. DOI: 10.3390/ijms21165880.

Maternal spindle transfer overcomes embryo developmental arrest caused by ooplasmic defects in mice.

Costa-Borges N, Spath K, Miguel-Escalada I, Mestres E, Balmaseda R, Serafin A Elife. 2020; 9.

PMID: 32347793 PMC: 7259950. DOI: 10.7554/eLife.48591.

Overgrowth of mice generated from postovulatory-aged oocyte spindles.

Shiina K, Komatsu M, Yokoi F, Bai H, Takahashi M, Kawahara M FASEB Bioadv. 2020; 1(7):393-403.

PMID: 32123841 PMC: 6996386. DOI: 10.1096/fba.2019-00005.

Tubulointerstitial nephritis antigen-like 1 deficiency alleviates age-dependent depressed ovulation associated with ovarian collagen deposition in mice.

Akaiwa M, Fukui E, Matsumoto H Reprod Med Biol. 2020; 19(1):50-57.

PMID: 31956285 PMC: 6955583. DOI: 10.1002/rmb2.12301.

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