Donor Oocyte Cytoplasmic Transfer Did Not Enhance Implantation of Embryos of Women with Poor Ovarian Reserve

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2002 May 15

PMID 12005304

Citations 2

Authors

M S Opsahl

L P Thorsell

M E Geltinger

M A Iwaszko

K L Blauer

R J Sherins

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine whether donor oocyte cytoplasm transferred into the oocytes of women < or = 40 years or with diminished ovarian reserve would enhance embryo quality, implantation, or pregnancy rates.

Methods: Study subjects included women > or = 40 years (15) or with abnormal FSH levels (3). Healthy volunteers (18) produced oocytes for cryopreservation. Donor oocytes were thawed and cytoplasm from surviving oocytes was injected with a single sperm into the cytoplasm of recipient oocytes. Outcome measures included embryo quality scores, implantation, and pregnancy rates.

Results: Eighteen donors produced 213 oocytes for cryopreservation and 39/171 (22.8%) survived thawing. Eighteen recipients initiated 25 IVF cycles with embryo transfer in 20 cycles after cytoplasmic transfer (CT). Four cycles resulted in three biochemical losses and one aneuploid clinical loss. Embryo quality did not improve with CT compared to pre-CT IVF cycles in six recipients.

Conclusions: CT with cryopreserved donor oocyte cytoplasm did not enhance success in women with advanced reproductive age or low ovarian reserve.

Citing Articles

Ooplasmic transfer in human oocytes: efficacy and concerns in assisted reproduction.

Darbandi S, Darbandi M, Khorshid H, Sadeghi M, Agarwal A, Sengupta P Reprod Biol Endocrinol. 2017; 15(1):77.

PMID: 28969648 PMC: 5625659. DOI: 10.1186/s12958-017-0292-z.

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes.

Mitsui A, Yoshizawa M, Matsumoto H, Fukui E J Assist Reprod Genet. 2008; 26(1):35-9.

PMID: 19096925 PMC: 2649337. DOI: 10.1007/s10815-008-9282-6.

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