Over-pressure Suppresses Ultrasonic-induced Drug Uptake

Overview

Journal Ultrasound Med Biol

Specialty Radiology

Date 2008 Dec 6

PMID 19056161

Citations 7

Authors

S Briant Stringham

Maria A Viskovska

Eric S Richardson

Seiga Ohmine

Ghaleb A Husseini

Byron K Murray

William G Pitt

Affiliations

Soon will be listed here.

Abstract

Ultrasound (US) is used to enhance and target delivery of drugs and genes to cancer tissues. The present study further examines the role of acoustic cavitation in US-induced permeabilization of cell membranes and subsequent drug or gene uptake by the cell. Rat colon cancer cells were exposed to ultrasound at various static pressures to examine the hypothesis that oscillating bubbles, also known as cavitating bubbles, permeabilize cells. Increasing pressure suppresses bubble cavitation activity; thus, if applied pressure were to reduce drug uptake, cell permeabilization would be strongly linked to bubble cavitation activity. Cells were exposed to 476 kHz pulsed ultrasound at average intensities of 2.75 W/cm(2) and 5.5 W/cm(2) at various pressures and times in an isothermal chamber. Cell fractions with reversible membrane damage (calcein uptake) and irreversible damage (propidium iodide uptake) were analyzed by flow cytometry. Pressurization to 3 atm nearly eliminated the biological effect of US in promoting calcein uptake. Data also showed a linear increase in membrane permeability with respect to insonation time and intensity. This research shows that US-mediated cell membrane permeability is likely linked to cavitation bubble activity.

Citing Articles

Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes.

Awad N, Paul V, AlSawaftah N, Husseini G Sci Rep. 2023; 13(1):16644.

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Harnessing Ultrasound for Targeting Drug Delivery to the Brain and Breaching the Blood-Brain Tumour Barrier.

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Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells.

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Ultrasound-triggered herceptin liposomes for breast cancer therapy.

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Kinetics and Thermodynamics of Acoustic Release of Doxorubicin from Non-stabilized polymeric Micelles.

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