A Family of LIC Vectors for High-throughput Cloning and Purification of Proteins

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2008 Nov 7

PMID 18988021

Citations 178

Authors

William H Eschenfeldt

Stols Lucy

Cynthia Sanville Millard

Andrzej Joachimiak

I Donnelly Mark

Affiliations

Soon will be listed here.

Abstract

Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate pro tein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we describe here.

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