High-throughput Process Development from Gene Cloning to Protein Production

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2023 Sep 15

PMID 37715258

Authors

Manman Sun

Alex Xiong Gao

Xiuxia Liu

Yankun Yang

Rodrigo Ledesma-Amaro

Zhonghu Bai

Affiliations

Soon will be listed here.

Abstract

In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.

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