Sequence of a Cellulase Gene from the Rumen Anaerobe Ruminococcus Flavefaciens 17

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Aug 1

PMID 1886616

Citations 4

Authors

C Cunningham

C A McPherson

J Martin

W J Harris

H J Flint

Affiliations

Soon will be listed here.

Abstract

A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethyl-cellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

Citing Articles

EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.

Rincon M, McCrae S, Kirby J, Scott K, Flint H Appl Environ Microbiol. 2001; 67(10):4426-31.

PMID: 11571138 PMC: 93185. DOI: 10.1128/AEM.67.10.4426-4431.2001.

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

Henrissat B, Bairoch A Biochem J. 1993; 293 ( Pt 3):781-8.

PMID: 8352747 PMC: 1134435. DOI: 10.1042/bj2930781.

Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens.

Zhang J, Martin J, Flint H Mol Gen Genet. 1994; 245(2):260-4.

PMID: 7816035 DOI: 10.1007/BF00283275.

Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria.

Whitehead T Curr Microbiol. 1993; 27(1):27-33.

PMID: 7763664 DOI: 10.1007/BF01576830.

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