New Families in the Classification of Glycosyl Hydrolases Based on Amino Acid Sequence Similarities

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Aug 1

PMID 8352747

Citations 437

Authors

B Henrissat

A Bairoch

Affiliations

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Abstract

301 glycosyl hydrolases and related enzymes corresponding to 39 EC entries of the I.U.B. classification system have been classified into 35 families on the basis of amino-acid-sequence similarities [Henrissat (1991) Biochem. J. 280, 309-316]. Approximately half of the families were found to be monospecific (containing only one EC number), whereas the other half were found to be polyspecific (containing at least two EC numbers). A > 60% increase in sequence data for glycosyl hydrolases (181 additional enzymes or enzyme domains sequences have since become available) allowed us to update the classification not only by the addition of more members to already identified families, but also by the finding of ten new families. On the basis of a comparison of 482 sequences corresponding to 52 EC entries, 45 families, out of which 22 are polyspecific, can now be defined. This classification has been implemented in the SWISS-PROT protein sequence data bank.

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References

Rouvinen J, Bergfors T, Teeri T, Knowles J, Jones T . Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science. 1990; 249(4967):380-6. DOI: 10.1126/science.2377893. View

Sumitomo N, Ozaki K, Kawai S, Ito S . Nucleotide sequence of the gene for an alkaline endoglucanase from an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis. Biosci Biotechnol Biochem. 1992; 56(6):872-7. DOI: 10.1271/bbb.56.872. View

Tsujibo H, Sakamoto T, Miyamoto K, Hasegawa T, Fujimoto M, Inamori Y . Amino acid compositions and partial sequences of xylanases from a new subspecies, Nocardiopsis dassonvillei subsp. alba OPC-18. Agric Biol Chem. 1991; 55(8):2173-4. View

Bairoch A . PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res. 1992; 20 Suppl:2013-8. PMC: 333978. DOI: 10.1093/nar/20.suppl.2013. View

Zverlov V, Laptev D, Tishkov V, Velikodvorskaja G . Nucleotide sequence of the Clostridium thermocellum laminarinase gene. Biochem Biophys Res Commun. 1991; 181(2):507-12. DOI: 10.1016/0006-291x(91)91217-z. View

Lao G, Ghangas G, JUNG E, Wilson D . DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca. J Bacteriol. 1991; 173(11):3397-407. PMC: 207951. DOI: 10.1128/jb.173.11.3397-3407.1991. View

Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon J . Cellulase families revealed by hydrophobic cluster analysis. Gene. 1989; 81(1):83-95. DOI: 10.1016/0378-1119(89)90339-9. View

Igarashi K, Ara K, Saeki K, Ozaki K, Kawai S, Ito S . Nucleotide sequence of the gene that encodes a neopullulanase from an alkalophilic Bacillus. Biosci Biotechnol Biochem. 1992; 56(3):514-6. DOI: 10.1271/bbb.56.514. View

Cutfield S, Brooke G, Sullivan P, Cutfield J . Crystallization of the exo(1,3)-beta-glucanase from Candida albicans. J Mol Biol. 1992; 225(1):217-8. DOI: 10.1016/0022-2836(92)91037-p. View

10.

Poch O, LHote H, Dallery V, Debeaux F, Fleer R, Sodoyer R . Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis. Gene. 1992; 118(1):55-63. DOI: 10.1016/0378-1119(92)90248-n. View

11.

Stoltzfus L, Sosa-Pineda B, Moskowitz S, Menon K, Dlott B, Hooper L . Cloning and characterization of cDNA encoding canine alpha-L-iduronidase. mRNA deficiency in mucopolysaccharidosis I dog. J Biol Chem. 1992; 267(10):6570-5. View

12.

Rixon J, Ferreira L, Durrant A, Laurie J, Hazlewood G, Gilbert H . Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa. Biochem J. 1992; 285 ( Pt 3):947-55. PMC: 1132887. DOI: 10.1042/bj2850947. View

13.

Py B, Haiech J, Chippaux M, Barras F . Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis. Protein Eng. 1991; 4(3):325-33. DOI: 10.1093/protein/4.3.325. View

14.

Cunningham C, McPherson C, Martin J, Harris W, Flint H . Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17. Mol Gen Genet. 1991; 228(1-2):320-3. DOI: 10.1007/BF00282484. View

15.

Castresana C, De Carvalho F, Gheysen G, Habets M, Inze D, Van Montagu M . Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia beta-1,3-glucanase gene. Plant Cell. 1990; 2(12):1131-43. PMC: 159961. DOI: 10.1105/tpc.2.12.1131. View

16.

Claeyssens M, Henrissat B . Specificity mapping of cellulolytic enzymes: classification into families of structurally related proteins confirmed by biochemical analysis. Protein Sci. 1992; 1(10):1293-7. PMC: 2142096. DOI: 10.1002/pro.5560011008. View

17.

Utt E, Eddy C, Keshav K, Ingram L . Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities. Appl Environ Microbiol. 1991; 57(4):1227-34. PMC: 182873. DOI: 10.1128/aem.57.4.1227-1234.1991. View

18.

Baird S, Hefford M, Johnson D, Sung W, Yaguchi M, Seligy V . The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo-beta-1,4-glucanases is essential for enzymatic activity. Biochem Biophys Res Commun. 1990; 169(3):1035-9. DOI: 10.1016/0006-291x(90)91998-8. View

19.

Leah R, Tommerup H, Svendsen I, Mundy J . Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem. 1991; 266(3):1564-73. View

20.

Croux C, Garcia J . Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes. Gene. 1991; 104(1):25-31. DOI: 10.1016/0378-1119(91)90460-s. View