Molecular Cloning of Genes from Ruminococcus Flavefaciens Encoding Xylanase and Beta(1-3,1-4)glucanase Activities

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1989 May 1

PMID 2757382

Citations 26

Authors

H J Flint

C A McPherson

J Bisset

Affiliations

Soon will be listed here.

Abstract

Clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of Ruminococcus flavefaciens 17 DNA made in bacteriophage lambda EMBL3. Hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. One of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities.

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References

Frischauf A, Lehrach H, Poustka A, Murray N . Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983; 170(4):827-42. DOI: 10.1016/s0022-2836(83)80190-9. View

Rasmussen M, Hespell R, White B, Bothast R . Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens. Appl Environ Microbiol. 1988; 54(4):890-7. PMC: 202569. DOI: 10.1128/aem.54.4.890-897.1988. View

Bryant M . Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972; 25(12):1324-8. DOI: 10.1093/ajcn/25.12.1324. View

Panbangred W, Kondo T, Negoro S, Shinmyo A, Okada H . Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli. Mol Gen Genet. 1983; 192(3):335-41. DOI: 10.1007/BF00392172. View

Howard G, White B . Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda. Appl Environ Microbiol. 1988; 54(7):1752-5. PMC: 202740. DOI: 10.1128/aem.54.7.1752-1755.1988. View

Lever M . Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): effect of bismuth on the reaction. Anal Biochem. 1977; 81(1):21-7. DOI: 10.1016/0003-2697(77)90594-2. View

Gilkes N, Langsford M, Kilburn D, Miller Jr R, Warren R . Mode of action and substrate specificities of cellulases from cloned bacterial genes. J Biol Chem. 1984; 259(16):10455-9. View

Wong K, Tan L, Saddler J . Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev. 1988; 52(3):305-17. PMC: 373146. DOI: 10.1128/mr.52.3.305-317.1988. View

Saito H, MIURA K . PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963; 72:619-29. View

10.

Teather R, Wood P . Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol. 1982; 43(4):777-80. PMC: 241917. DOI: 10.1128/aem.43.4.777-780.1982. View

11.

Barros M, Thomson J . Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens. J Bacteriol. 1987; 169(4):1760-2. PMC: 212014. DOI: 10.1128/jb.169.4.1760-1762.1987. View

12.

Lee S, Forsberg C, Rattray J . Purification and Characterization of Two Endoxylanases from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1987; 53(4):644-50. PMC: 203729. DOI: 10.1128/aem.53.4.644-650.1987. View

13.

Irvin J, Teather R . Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli. Appl Environ Microbiol. 1988; 54(11):2672-6. PMC: 204354. DOI: 10.1128/aem.54.11.2672-2676.1988. View

14.

Gardner R, Doerner K, White B . Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1. J Bacteriol. 1987; 169(10):4581-8. PMC: 213825. DOI: 10.1128/jb.169.10.4581-4588.1987. View

15.

Flint H, Thomson A, Bisset J . Plasmid-associated transfer of tetracycline resistance in Bacteroides ruminicola. Appl Environ Microbiol. 1988; 54(4):855-60. PMC: 202563. DOI: 10.1128/aem.54.4.855-860.1988. View