Adult SVZ Stem Cells Lie in a Vascular Niche: a Quantitative Analysis of Niche Cell-cell Interactions

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2008 Sep 13

PMID 18786416

Citations 518

Authors

Qin Shen

Yue Wang

Erzsebet Kokovay

Gang Lin

Shu-Mien Chuang

Susan K Goderie

Badrinath Roysam

Sally Temple

Affiliations

Soon will be listed here.

Abstract

There is an emerging understanding of the importance of the vascular system within stem cell niches. Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor alpha6beta1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche.

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References

Li A, Simmons P, Kaur P . Identification and isolation of candidate human keratinocyte stem cells based on cell surface phenotype. Proc Natl Acad Sci U S A. 1998; 95(7):3902-7. PMC: 19935. DOI: 10.1073/pnas.95.7.3902. View

Capela A, Temple S . LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal. Neuron. 2002; 35(5):865-75. DOI: 10.1016/s0896-6273(02)00835-8. View

Kerever A, Schnack J, Vellinga D, Ichikawa N, Moon C, Arikawa-Hirasawa E . Novel extracellular matrix structures in the neural stem cell niche capture the neurogenic factor fibroblast growth factor 2 from the extracellular milieu. Stem Cells. 2007; 25(9):2146-57. DOI: 10.1634/stemcells.2007-0082. View

Simard M, Arcuino G, Takano T, Liu Q, Nedergaard M . Signaling at the gliovascular interface. J Neurosci. 2003; 23(27):9254-62. PMC: 6740832. View

Wichterle H, Garcia-Verdugo J, Alvarez-Buylla A . Direct evidence for homotypic, glia-independent neuronal migration. Neuron. 1997; 18(5):779-91. DOI: 10.1016/s0896-6273(00)80317-7. View

Lin G, Bjornsson C, Smith K, Abdul-Karim M, Turner J, Shain W . Automated image analysis methods for 3-D quantification of the neurovascular unit from multichannel confocal microscope images. Cytometry A. 2005; 66(1):9-23. DOI: 10.1002/cyto.a.20149. View

Breier G, Albrecht U, Sterrer S, Risau W . Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development. 1992; 114(2):521-32. DOI: 10.1242/dev.114.2.521. View

Conover J, Doetsch F, Garcia-Verdugo J, Gale N, Yancopoulos G, Alvarez-Buylla A . Disruption of Eph/ephrin signaling affects migration and proliferation in the adult subventricular zone. Nat Neurosci. 2000; 3(11):1091-7. DOI: 10.1038/80606. View

Brightman M . The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. I. Ependymal distribution. J Cell Biol. 1965; 26(1):99-123. PMC: 2106700. DOI: 10.1083/jcb.26.1.99. View

10.

Doetsch F, Petreanu L, Caille I, Garcia-Verdugo J, Alvarez-Buylla A . EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron. 2002; 36(6):1021-34. DOI: 10.1016/s0896-6273(02)01133-9. View

11.

Weinstein B . Vessels and nerves: marching to the same tune. Cell. 2005; 120(3):299-302. DOI: 10.1016/j.cell.2005.01.010. View

12.

Gilbertson R, Rich J . Making a tumour's bed: glioblastoma stem cells and the vascular niche. Nat Rev Cancer. 2007; 7(10):733-6. DOI: 10.1038/nrc2246. View

13.

Alvarez-Buylla A, Garcia-Verdugo J, Mateo A, Merchant-Larios H . Primary neural precursors and intermitotic nuclear migration in the ventricular zone of adult canaries. J Neurosci. 1998; 18(3):1020-37. PMC: 6792779. View

14.

Baker K, Daniels S, Lennington J, Lardaro T, Czap A, Notti R . Neuroblast protuberances in the subventricular zone of the regenerative MRL/MpJ mouse. J Comp Neurol. 2006; 498(6):747-61. DOI: 10.1002/cne.21090. View

15.

Fortunel N, Otu H, Ng H, Chen J, Mu X, Chevassut T . Comment on " 'Stemness': transcriptional profiling of embryonic and adult stem cells" and "a stem cell molecular signature". Science. 2003; 302(5644):393. DOI: 10.1126/science.1086384. View

16.

STRONG L . THE EARLY EMBRYONIC PATTERN OF INTERNAL VASCULARIZATION OF THE MAMMALIAN CEREBRAL CORTEX. J Comp Neurol. 1964; 123:121-38. DOI: 10.1002/cne.901230111. View

17.

Dorr A, Sled J, Kabani N . Three-dimensional cerebral vasculature of the CBA mouse brain: a magnetic resonance imaging and micro computed tomography study. Neuroimage. 2007; 35(4):1409-23. DOI: 10.1016/j.neuroimage.2006.12.040. View

18.

Louissaint Jr A, Rao S, Leventhal C, Goldman S . Coordinated interaction of neurogenesis and angiogenesis in the adult songbird brain. Neuron. 2002; 34(6):945-60. DOI: 10.1016/s0896-6273(02)00722-5. View

19.

Emsley J, Hagg T . alpha6beta1 integrin directs migration of neuronal precursors in adult mouse forebrain. Exp Neurol. 2003; 183(2):273-85. DOI: 10.1016/s0014-4886(03)00209-7. View

20.

Fuchs E, Tumbar T, Guasch G . Socializing with the neighbors: stem cells and their niche. Cell. 2004; 116(6):769-78. DOI: 10.1016/s0092-8674(04)00255-7. View