Deconstructing Human Embryonic Stem Cell Cultures: Niche Regulation of Self-renewal and Pluripotency

Overview

Journal J Mol Med (Berl)

Specialty General Medicine

Date 2008 Jun 4

PMID 18521556

Citations 22

Authors

Morag H Stewart

Sean C Bendall

Mickie Bhatia

Affiliations

Soon will be listed here.

Abstract

The factors and signaling pathways controlling pluripotent human cell properties, both embryonic and induced, have not been fully investigated. Failure to account for functional heterogeneity within human embryonic stem cell (hESC) cultures has led to inconclusive results in previous work examining extrinsic influences governing hESC fate (self renewal vs. differentiation vs. death). Here, we attempt to reconcile these inconsistencies with recent reports demonstrating that an autologously produced in vitro niche regulates hESCs. Moreover, we focus on the reciprocal paracrine signals within the in vitro hESC niche allowing for the maintenance and/or expansion of the hESC colony-initiating cell (CIC). Based on this, it is clear that separation of hESC-CICs, apart from their differentiated derivatives, will be essential in future studies involving their molecular regulation. Understanding how extrinsic factors control hESC self-renewal and differentiation will allow us to culture and differentiate these pluripotent cells with higher efficiency. This knowledge will be essential for clinical applications using human pluripotent cells in regenerative medicine.

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References

Crittenden S, Bernstein D, Bachorik J, Thompson B, Gallegos M, Petcherski A . A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans. Nature. 2002; 417(6889):660-3. DOI: 10.1038/nature754. View

Becker A, McCulloch E, Till J . Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature. 1963; 197:452-4. DOI: 10.1038/197452a0. View

Xu R, Peck R, Li D, Feng X, Ludwig T, Thomson J . Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods. 2005; 2(3):185-90. DOI: 10.1038/nmeth744. View

Tulina N, Matunis E . Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling. Science. 2001; 294(5551):2546-9. DOI: 10.1126/science.1066700. View

Stewart M, Bosse M, Chadwick K, Menendez P, Bendall S, Bhatia M . Clonal isolation of hESCs reveals heterogeneity within the pluripotent stem cell compartment. Nat Methods. 2006; 3(10):807-15. DOI: 10.1038/nmeth939. View

Xu R, Chen X, Li D, Li R, Addicks G, Glennon C . BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol. 2002; 20(12):1261-4. DOI: 10.1038/nbt761. View

Lei T, Jacob S, Ajil-Zaraa I, Dubuisson J, Irion O, Jaconi M . Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges. Cell Res. 2007; 17(8):682-8. DOI: 10.1038/cr.2007.61. View

Bigdeli N, Andersson M, Strehl R, Emanuelsson K, Kilmare E, Hyllner J . Adaptation of human embryonic stem cells to feeder-free and matrix-free culture conditions directly on plastic surfaces. J Biotechnol. 2007; 133(1):146-53. DOI: 10.1016/j.jbiotec.2007.08.045. View

Klimanskaya I, Chung Y, Becker S, Lu S, Lanza R . Human embryonic stem cell lines derived from single blastomeres. Nature. 2006; 444(7118):481-5. DOI: 10.1038/nature05142. View

10.

Armstrong L, Hughes O, Yung S, Hyslop L, Stewart R, Wappler I . The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis. Hum Mol Genet. 2006; 15(11):1894-913. DOI: 10.1093/hmg/ddl112. View

11.

Skottman H, Hovatta O . Culture conditions for human embryonic stem cells. Reproduction. 2006; 132(5):691-8. DOI: 10.1530/rep.1.01079. View

12.

Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk G . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nat Biotechnol. 2004; 22(6):707-16. DOI: 10.1038/nbt971. View

13.

Prowse A, McQuade L, Bryant K, Marcal H, Gray P . Identification of potential pluripotency determinants for human embryonic stem cells following proteomic analysis of human and mouse fibroblast conditioned media. J Proteome Res. 2007; 6(9):3796-807. DOI: 10.1021/pr0702262. View

14.

Draper J, Smith K, Gokhale P, Moore H, Maltby E, Johnson J . Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol. 2003; 22(1):53-4. DOI: 10.1038/nbt922. View

15.

Lee J, Song J, Lee J, Park J, Kim S, Kang S . Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction. 2004; 128(6):727-35. DOI: 10.1530/rep.1.00415. View

16.

Ginis I, Luo Y, Miura T, Thies S, Brandenberger R, Gerecht-Nir S . Differences between human and mouse embryonic stem cells. Dev Biol. 2004; 269(2):360-80. DOI: 10.1016/j.ydbio.2003.12.034. View

17.

Greber B, Lehrach H, Adjaye J . Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells. 2006; 25(2):455-64. DOI: 10.1634/stemcells.2006-0476. View

18.

Amit M, Itskovitz-Eldor J . Sources, derivation, and culture of human embryonic stem cells. Semin Reprod Med. 2006; 24(5):298-303. DOI: 10.1055/s-2006-954939. View

19.

James D, Levine A, Besser D, Hemmati-Brivanlou A . TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells. Development. 2005; 132(6):1273-82. DOI: 10.1242/dev.01706. View

20.

Mantel C, Guo Y, Lee M, Kim M, Han M, Shibayama H . Checkpoint-apoptosis uncoupling in human and mouse embryonic stem cells: a source of karyotpic instability. Blood. 2007; 109(10):4518-27. PMC: 1885509. DOI: 10.1182/blood-2006-10-054247. View