Aptamer-targeted Gold Nanoparticles As Molecular-specific Contrast Agents for Reflectance Imaging

Overview

Journal Bioconjug Chem

Publisher American Chemical Society

Specialty Biochemistry

Date 2008 Jun 3

PMID 18512972

Citations 58

Authors

David J Javier

Nitin Nitin

Matthew Levy

Andrew Ellington

Rebecca Richards-Kortum

Affiliations

Soon will be listed here.

Abstract

Targeted metallic nanoparticles have shown potential as a platform for development of molecular-specific contrast agents. Aptamers have recently been demonstrated as ideal candidates for molecular targeting applications. In this study, we investigated the development of aptamer-based gold nanoparticles as contrast agents, using aptamers as targeting agents and gold nanoparticles as imaging agents. We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles. The chemical and optical properties of the aptamer-gold conjugates were characterized using size measurements and oligonucleotide quantitation assays. We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(-) cell lines treated with the anti-PSMA aptamer-gold conjugates. This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.

Citing Articles

Formulation optimization of lyophilized aptamer-gold nanoparticles: Maintained colloidal stability and cellular uptake.

Saidi D, Obeidat M, Alsotari S, Ibrahim A, Al-Buqain R, Wehaibi S Heliyon. 2024; 10(10):e30743.

PMID: 38774322 PMC: 11107208. DOI: 10.1016/j.heliyon.2024.e30743.

Measurement of Neuropeptide Y in Aptamer-Modified Planar Electrodes.

Lopez L, Martinez L, Caicedo J, Fernandez-Vega L, Cunci L Electrochim Acta. 2024; 488.

PMID: 38654828 PMC: 11034791. DOI: 10.1016/j.electacta.2024.144243.

Nucleic Acid Aptamer-Based Biosensors: A Review.

Sequeira-Antunes B, Ferreira H Biomedicines. 2023; 11(12).

PMID: 38137422 PMC: 10741014. DOI: 10.3390/biomedicines11123201.

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles.

Xu R, Ouyang L, Chen H, Zhang G, Zhe J Biosensors (Basel). 2023; 13(4).

PMID: 37185549 PMC: 10136534. DOI: 10.3390/bios13040474.

Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors.

Hu W, Wu Y, Vu C, Chen W Sensors (Basel). 2023; 23(2).

PMID: 36679421 PMC: 9865274. DOI: 10.3390/s23020625.

References

Chu T, Marks 3rd J, Lavery L, Faulkner S, Rosenblum M, Ellington A . Aptamer:toxin conjugates that specifically target prostate tumor cells. Cancer Res. 2006; 66(12):5989-92. DOI: 10.1158/0008-5472.CAN-05-4583. View

Rodriguez J, Dvorak J, Jirsak T, Liu G, Hrbek J, Aray Y . Coverage effects and the nature of the metal-sulfur bond in S/Au(111): high-resolution photoemission and density-functional studies. J Am Chem Soc. 2003; 125(1):276-85. DOI: 10.1021/ja021007e. View

Aslan K, Lakowicz J, Geddes C . Nanogold-plasmon-resonance-based glucose sensing. Anal Biochem. 2004; 330(1):145-55. PMC: 6986337. DOI: 10.1016/j.ab.2004.03.032. View

Mirkin C, LETSINGER R, Mucic R, Storhoff J . A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature. 1996; 382(6592):607-9. DOI: 10.1038/382607a0. View

Brody E, Gold L . Aptamers as therapeutic and diagnostic agents. J Biotechnol. 2000; 74(1):5-13. DOI: 10.1016/s1389-0352(99)00004-5. View

Chu T, Shieh F, Lavery L, Levy M, Richards-Kortum R, Korgel B . Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. Biosens Bioelectron. 2006; 21(10):1859-66. DOI: 10.1016/j.bios.2005.12.015. View

Aaron J, Nitin N, Travis K, Kumar S, Collier T, Park S . Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo. J Biomed Opt. 2007; 12(3):034007. DOI: 10.1117/1.2737351. View

Aslan K, Zhang J, Lakowicz J, Geddes C . Saccharide sensing using gold and silver nanoparticles--a review. J Fluoresc. 2004; 14(4):391-400. PMC: 6874496. DOI: 10.1023/b:jofl.0000031820.17358.28. View

Bunka D, Stockley P . Aptamers come of age - at last. Nat Rev Microbiol. 2006; 4(8):588-96. DOI: 10.1038/nrmicro1458. View

10.

Adams K, Ke S, Kwon S, Liang F, Fan Z, Lu Y . Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer. J Biomed Opt. 2007; 12(2):024017. DOI: 10.1117/1.2717137. View

11.

Nitin N, Javier D, Roblyer D, Richards-Kortum R . Widefield and high-resolution reflectance imaging of gold and silver nanospheres. J Biomed Opt. 2007; 12(5):051505. DOI: 10.1117/1.2800314. View

12.

Lupold S, Hicke B, Lin Y, Coffey D . Identification and characterization of nuclease-stabilized RNA molecules that bind human prostate cancer cells via the prostate-specific membrane antigen. Cancer Res. 2002; 62(14):4029-33. View

13.

Hurst S, Lytton-Jean A, Mirkin C . Maximizing DNA loading on a range of gold nanoparticle sizes. Anal Chem. 2006; 78(24):8313-8. PMC: 2525614. DOI: 10.1021/ac0613582. View

14.

Sokolov K, Follen M, Aaron J, Pavlova I, Malpica A, Lotan R . Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles. Cancer Res. 2003; 63(9):1999-2004. View

15.

Medley C, Smith J, Tang Z, Wu Y, Bamrungsap S, Tan W . Gold nanoparticle-based colorimetric assay for the direct detection of cancerous cells. Anal Chem. 2008; 80(4):1067-72. DOI: 10.1021/ac702037y. View

16.

Nitin N, Javier D, Richards-Kortum R . Oligonucleotide-coated metallic nanoparticles as a flexible platform for molecular imaging agents. Bioconjug Chem. 2007; 18(6):2090-6. DOI: 10.1021/bc0701242. View