Disparate Proteome Reactivity Profiles of Carbon Electrophiles

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2008 May 20

PMID 18488014

Citations 96

Authors

Eranthie Weerapana

Gabriel M Simon

Benjamin F Cravatt

Affiliations

Soon will be listed here.

Abstract

Insights into the proteome reactivity of electrophiles are crucial for designing activity-based probes for enzymes lacking cognate affinity labels. Here, we show that different classes of carbon electrophiles exhibit markedly distinct amino acid labeling profiles in proteomes, ranging from selective reactivity with cysteine to adducts with several amino acids. These data specify electrophilic chemotypes with restricted and permissive reactivity profiles to guide the design of next-generation functional proteomics probes.

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