Establishment of the Culture Model System That Reflects the Process of Terminal Differentiation of Connective Tissue-type Mast Cells

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2008 Apr 3

PMID 18381075

Citations 17

Authors

Hirotsugu Takano

Shunsuke Nakazawa

Yasushi Okuno

Naritoshi Shirata

Sohken Tsuchiya

Takayuki Kainoh

Seigoh Takamatsu

Kazuyuki Furuta

Yoshitaka Taketomi

Yuko Naito

Hiromu Takematsu

Yasunori Kozutsumi

Gozoh Tsujimoto

Makoto Murakami

Ichiro Kudo

Atsushi Ichikawa

Kazuhisa Nakayama

Yukihiko Sugimoto

Satoshi Tanaka

Affiliations

Soon will be listed here.

Abstract

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

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