A Decade of Yeast Surface Display Technology: Where Are We Now?

Overview

Journal Comb Chem High Throughput Screen

Publisher Bentham Science Publishers

Specialty Chemistry

Date 2008 Mar 14

PMID 18336206

Citations 71

Authors

Lauren R Pepper

Yong Ku Cho

Eric T Boder

Eric V Shusta

Affiliations

Soon will be listed here.

Abstract

Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Recent advances have greatly expanded the capability of yeast surface display, and are highlighted by cell-based selections, epitope mapping, cDNA library screening, and cell adhesion engineering. In this review, we discuss the state-of-the-art yeast display methodologies and the rapidly expanding set of applications afforded by this technology.

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References

Graff C, Chester K, Begent R, Wittrup K . Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C. Protein Eng Des Sel. 2004; 17(4):293-304. DOI: 10.1093/protein/gzh038. View

Cochran J, Kim Y, Lippow S, Rao B, Wittrup K . Improved mutants from directed evolution are biased to orthologous substitutions. Protein Eng Des Sel. 2006; 19(6):245-53. DOI: 10.1093/protein/gzl006. View

Tanino T, Fukuda H, Kondo A . Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system. Biotechnol Prog. 2006; 22(4):989-93. DOI: 10.1021/bp060133+. View

Bhatia S, Swers J, Camphausen R, Wittrup K, Hammer D . Rolling adhesion kinematics of yeast engineered to express selectins. Biotechnol Prog. 2003; 19(3):1033-7. DOI: 10.1021/bp025756b. View

Rao B, Driver I, Lauffenburger D, Wittrup K . Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect. Mol Pharmacol. 2004; 66(4):864-9. DOI: 10.1124/mol.66.4.. View

Starwalt S, Masteller E, Bluestone J, Kranz D . Directed evolution of a single-chain class II MHC product by yeast display. Protein Eng. 2003; 16(2):147-56. DOI: 10.1093/proeng/gzg018. View

Dranginis A, Rauceo J, Coronado J, Lipke P . A biochemical guide to yeast adhesins: glycoproteins for social and antisocial occasions. Microbiol Mol Biol Rev. 2007; 71(2):282-94. PMC: 1899881. DOI: 10.1128/MMBR.00037-06. View

Schweickhardt R, Jiang X, Garone L, Brondyk W . Structure-expression relationship of tumor necrosis factor receptor mutants that increase expression. J Biol Chem. 2003; 278(31):28961-7. DOI: 10.1074/jbc.M212019200. View

Chung K, Nybakken G, Thompson B, Engle M, Marri A, Fremont D . Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms. J Virol. 2006; 80(3):1340-51. PMC: 1346945. DOI: 10.1128/JVI.80.3.1340-1351.2006. View

10.

Bidlingmaier S, Liu B . Construction and application of a yeast surface-displayed human cDNA library to identify post-translational modification-dependent protein-protein interactions. Mol Cell Proteomics. 2005; 5(3):533-40. DOI: 10.1074/mcp.M500309-MCP200. View

11.

Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K . Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004; 287(1-2):147-58. DOI: 10.1016/j.jim.2004.01.024. View

12.

Wang X, Cho Y, Shusta E . Mining a yeast library for brain endothelial cell-binding antibodies. Nat Methods. 2007; 4(2):143-5. PMC: 2637222. DOI: 10.1038/nmeth993. View

13.

Bowley D, Labrijn A, Zwick M, Burton D . Antigen selection from an HIV-1 immune antibody library displayed on yeast yields many novel antibodies compared to selection from the same library displayed on phage. Protein Eng Des Sel. 2007; 20(2):81-90. DOI: 10.1093/protein/gzl057. View

14.

Schreuder M, Brekelmans S, van den Ende H, Klis F . Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Yeast. 1993; 9(4):399-409. DOI: 10.1002/yea.320090410. View

15.

Holler P, Holman P, Shusta E, OHerrin S, Wittrup K, Kranz D . In vitro evolution of a T cell receptor with high affinity for peptide/MHC. Proc Natl Acad Sci U S A. 2000; 97(10):5387-92. PMC: 25838. DOI: 10.1073/pnas.080078297. View

16.

Jones L, Brophy S, Bankovich A, Colf L, Hanick N, Garcia K . Engineering and characterization of a stabilized alpha1/alpha2 module of the class I major histocompatibility complex product Ld. J Biol Chem. 2006; 281(35):25734-44. DOI: 10.1074/jbc.M604343200. View

17.

Holler P, Kranz D . Quantitative analysis of the contribution of TCR/pepMHC affinity and CD8 to T cell activation. Immunity. 2003; 18(2):255-64. DOI: 10.1016/s1074-7613(03)00019-0. View

18.

Kim Y, Bhandari R, Cochran J, Kuriyan J, Wittrup K . Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins. 2005; 62(4):1026-35. DOI: 10.1002/prot.20618. View

19.

Lee H, Lee S, Park K, Kim J, Kwon M, Kim Y . Construction and characterization of a pseudo-immune human antibody library using yeast surface display. Biochem Biophys Res Commun. 2006; 346(3):896-903. DOI: 10.1016/j.bbrc.2006.05.202. View

20.

Feldhaus M, Siegel R, Opresko L, Coleman J, Feldhaus J, Yeung Y . Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol. 2003; 21(2):163-70. DOI: 10.1038/nbt785. View