The V Beta Repertoire of Mouse Gut Homodimeric Alpha CD8+ Intraepithelial T Cell Receptor Alpha/beta + Lymphocytes Reveals a Major Extrathymic Pathway of T Cell Differentiation

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1991 Feb 1

PMID 1824858

Citations 94

Authors

B Rocha

P Vassalli

Affiliations

Soon will be listed here.

Abstract

Gut intraepithelial lymphocytes (IEL) contain two independent T cell receptor alpha/beta + T cell populations, with different V beta repertoires. In DBA/2 mice (Mlsa, IE+), the CD4+ and heterodimeric alpha/beta CD8+ thymodependent T cell pool shows the same deletion of V beta 6, 8.1, and 11+ cells as found in peripheral lymphoid organs. In contrast, such deletions are not observed in the pool of IEL bearing homodimeric alpha CD8+ chains, in which these V beta families are frequently observed in high amounts. The size of this gut homodimeric alpha CD8+ IEL pool and its different V beta repertoire selection demonstrate the existence of a major extrathymic pathway of T cell differentiation with a gut-restricted localization. The large amount of the thymo-independent, homodimeric alpha CD8+ IEL found in the small bowel may contribute to a first line of defense against exogenous superantigens.

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References

PAPIERNIK M, Pontoux C . In vivo and in vitro repertoire of CD3+CD4-CD8- thymocytes. Int Immunol. 1990; 2(5):407-12. DOI: 10.1093/intimm/2.5.407. View

Zatz M, Lance E . The distribution of chromium 51-labelled lymphoid cells in the mouse. A survey of anatomical compartments. Cell Immunol. 1970; 1(1):3-17. DOI: 10.1016/0008-8749(70)90057-2. View

Kubo R, Born W, Kappler J, Marrack P, Pigeon M . Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-42. View

Blanc D, Bron C, Gabert J, Letourneur F, MacDonald H, Malissen B . Gene transfer of the Ly-3 chain gene of the mouse CD8 molecular complex: co-transfer with the Ly-2 polypeptide gene results in detectable cell surface expression of the Ly-3 antigenic determinants. Eur J Immunol. 1988; 18(4):613-9. DOI: 10.1002/eji.1830180419. View

MacDonald H, Lees R, Bron C, Sordat B, MIESCHER G . T cell antigen receptor expression in athymic (nu/nu) mice. Evidence for an oligoclonal beta chain repertoire. J Exp Med. 1987; 166(1):195-209. PMC: 2188643. DOI: 10.1084/jem.166.1.195. View

Goodman T, Lefrancois L . Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989; 170(5):1569-81. PMC: 2189511. DOI: 10.1084/jem.170.5.1569. View

Bill J, Kanagawa O, Woodland D, Palmer E . The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells. J Exp Med. 1989; 169(4):1405-19. PMC: 2189240. DOI: 10.1084/jem.169.4.1405. View

Gao E, Kanagawa O, Sprent J . Capacity of unprimed CD4+ and CD8+ T cells expressing V beta 11 receptors to respond to I-E alloantigens in vivo. J Exp Med. 1989; 170(6):1947-57. PMC: 2189546. DOI: 10.1084/jem.170.6.1947. View

Cron R, Gajewski T, Sharrow S, Fitch F, Matis L, Bluestone J . Phenotypic and functional analysis of murine CD3+,CD4-,CD8- TCR-gamma delta-expressing peripheral T cells. J Immunol. 1989; 142(11):3754-62. View

10.

Fry A, Jones L, Kruisbeek A, Matis L . Thymic requirement for clonal deletion during T cell development. Science. 1989; 246(4933):1044-6. DOI: 10.1126/science.2511630. View

11.

Rocha B . Characterization of V beta-bearing cells in athymic (nu/nu) mice suggests an extrathymic pathway for T cell differentiation. Eur J Immunol. 1990; 20(4):919-25. DOI: 10.1002/eji.1830200430. View

12.

MacDonald H, Schreyer M, Howe R, Bron C . Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells. Eur J Immunol. 1990; 20(4):927-30. DOI: 10.1002/eji.1830200431. View

13.

von Boehmer H . Developmental biology of T cells in T cell-receptor transgenic mice. Annu Rev Immunol. 1990; 8:531-56. DOI: 10.1146/annurev.iy.08.040190.002531. View

14.

Marrack P, Kappler J . The staphylococcal enterotoxins and their relatives. Science. 1990; 248(4956):705-11. DOI: 10.1126/science.2185544. View

15.

Dialynas D, Quan Z, Wall K, Pierres A, Quintans J, Loken M . Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983; 131(5):2445-51. View

16.

Griscelli C, Vassalli P . The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978; 148(6):1661-77. PMC: 2185102. DOI: 10.1084/jem.148.6.1661. View

17.

Maleckar J, Sherman L . The composition of the T cell receptor repertoire in nude mice. J Immunol. 1987; 138(11):3873-6. View