NSOM/QD-based Nanoscale Immunofluorescence Imaging of Antigen-specific T-cell Receptor Responses During an in Vivo Clonal Vγ2Vδ2 T-cell Expansion

Overview

Journal Blood

Publisher Elsevier

Specialty Hematology

Date 2007 Nov 28

PMID 18039956

Citations 23

Authors

Yong Chen

Lingyun Shao

Zahida Ali

Jiye Cai

Zheng W Chen

Affiliations

Soon will be listed here.

Abstract

Nanoscale imaging of an in vivo antigen-specific T-cell immune response has not been reported. Here, the combined near-field scanning optical microscopy- and fluorescent quantum dot-based nanotechnology was used to perform immunofluorescence imaging of antigen-specific T-cell receptor (TCR) response in an in vivo model of clonal T-cell expansion. The near-field scanning optical microscopy/quantum dot system provided a best-optical-resolution (<50 nm) nano-scale imaging of Vgamma2Vdelta2 TCR on the membrane of nonstimulated Vgamma2Vdelta2 T cells. Before Ag-induced clonal expansion, these nonstimulating Vgamma2Vdelta2 TCRs appeared to be distributed differently from their alphabeta TCR counterparts on the cell surface. Surprisingly, Vgamma2Vdelta2 TCR nanoclusters not only were formed but also sustained on the membrane during an in vivo clonal expansion of Vgamma2Vdelta2 T cells after phosphoantigen treatment or phosphoantigen plus mycobacterial infection. The TCR nanoclusters could array to form nanodomains or microdomains on the membrane of clonally expanded Vgamma2Vdelta2 T cells. Interestingly, expanded Vgamma2Vdelta2 T cells bearing TCR nanoclusters or nanodomains were able to rerecognize phosphoantigen and to exert better effector function. These studies provided nanoscale insight into the in vivo T-cell immune response.

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References

Campi G, Varma R, Dustin M . Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling. J Exp Med. 2005; 202(8):1031-6. PMC: 1373686. DOI: 10.1084/jem.20051182. View

Anikeeva N, Lebedeva T, Clapp A, Goldman E, Dustin M, Mattoussi H . Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. Proc Natl Acad Sci U S A. 2006; 103(45):16846-51. PMC: 1636542. DOI: 10.1073/pnas.0607771103. View

Gerton J, Wade L, Lessard G, Ma Z, Quake S . Tip-enhanced fluorescence microscopy at 10 nanometer resolution. Phys Rev Lett. 2004; 93(18):180801. DOI: 10.1103/PhysRevLett.93.180801. View

Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind R, Kollas A . Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli. FEBS Lett. 2001; 509(2):317-22. DOI: 10.1016/s0014-5793(01)03191-x. View

Rasmussen A, Deckert V . New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy. Anal Bioanal Chem. 2004; 381(1):165-72. DOI: 10.1007/s00216-004-2896-3. View

Wang H, Lee H, Bukowski J, Li H, Mariuzza R, Chen Z . Conservation of nonpeptide antigen recognition by rhesus monkey V gamma 2V delta 2 T cells. J Immunol. 2003; 170(7):3696-706. DOI: 10.4049/jimmunol.170.7.3696. View

Wu X, Liu H, Liu J, Haley K, Treadway J, Larson J . Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol. 2002; 21(1):41-6. DOI: 10.1038/nbt764. View

Chen Y, Cai J, Zhao T, Wang C, Dong S, Luo S . Atomic force microscopy imaging and 3-D reconstructions of serial thin sections of a single cell and its interior structures. Ultramicroscopy. 2005; 103(3):173-82. PMC: 2873076. DOI: 10.1016/j.ultramic.2004.11.019. View

Van Stipdonk M, Lemmens E, Schoenberger S . Naïve CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation. Nat Immunol. 2001; 2(5):423-9. DOI: 10.1038/87730. View

10.

Gao X, Cui Y, Levenson R, Chung L, Nie S . In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol. 2004; 22(8):969-76. DOI: 10.1038/nbt994. View

11.

Lee W, Pasos G, Cecchini L, Mittler J . Continued antigen stimulation is not required during CD4(+) T cell clonal expansion. J Immunol. 2002; 168(4):1682-9. DOI: 10.4049/jimmunol.168.4.1682. View

12.

Hoppener C, Siebrasse J, Peters R, Kubitscheck U, Naber A . High-resolution near-field optical imaging of single nuclear pore complexes under physiological conditions. Biophys J. 2005; 88(5):3681-8. PMC: 1305514. DOI: 10.1529/biophysj.104.051458. View

13.

Enderle T, Ha T, Ogletree D, Chemla D, Magowan C, Weiss S . Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy. Proc Natl Acad Sci U S A. 1997; 94(2):520-5. PMC: 19545. DOI: 10.1073/pnas.94.2.520. View

14.

Service R . Materials and biology. Nanotechnology takes aim at cancer. Science. 2005; 310(5751):1132-4. DOI: 10.1126/science.310.5751.1132. View

15.

Shen L, Shen Y, Huang D, Qiu L, Sehgal P, Du G . Development of Vgamma2Vdelta2+ T cell responses during active mycobacterial coinfection of simian immunodeficiency virus-infected macaques requires control of viral infection and immune competence of CD4+ T cells. J Infect Dis. 2004; 190(8):1438-47. PMC: 2865241. DOI: 10.1086/423939. View

16.

Novotny L, Stranick S . Near-field optical microscopy and spectroscopy with pointed probes. Annu Rev Phys Chem. 2006; 57:303-31. DOI: 10.1146/annurev.physchem.56.092503.141236. View

17.

Hayes S, Love P . Stoichiometry of the murine gammadelta T cell receptor. J Exp Med. 2006; 203(1):47-52. PMC: 2118071. DOI: 10.1084/jem.20051886. View

18.

Larson D, Zipfel W, Williams R, Clark S, Bruchez M, Wise F . Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science. 2003; 300(5624):1434-6. DOI: 10.1126/science.1083780. View

19.

Yokosuka T, Sakata-Sogawa K, Kobayashi W, Hiroshima M, Hashimoto-Tane A, Tokunaga M . Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76. Nat Immunol. 2005; 6(12):1253-62. DOI: 10.1038/ni1272. View

20.

Davis K, Abrams B, Iyer S, Hoffman R, Bishop J . Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation. Cytometry. 1998; 33(2):197-205. DOI: 10.1002/(sici)1097-0320(19981001)33:2<197::aid-cyto14>3.0.co;2-p. View