Is the Gramicidin a Transmembrane Channel Single-stranded or Double-stranded Helix? A Simple Unequivocal Determination

Overview

Journal Science

Specialty Science

Date 1983 Sep 9

PMID 17736655

Citations 24

Authors

D W Urry

T L Trapane

K U Prasad

Affiliations

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Abstract

Thallium ion-induced carbonyl carbon chemical shifts were compared for all of the L-residue-peptide carbonyl carbons of the gramicidin A transmembrane channel. Molecular structures were deduced by using the argument that helically equivalent and equally proximal carbonyls would exhibit essentially equivalent ion-induced chemical shifts. The transmembrane channel was found to be a head-to-head dimer with the structure of a left-handed, single-stranded beta-helix.

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