Efficient Gene Transfection Using Chitosan-alginate Core-shell Nanoparticles

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2007 Aug 29

PMID 17722533

Citations 8

Authors

Jin-Oh You

Yu-Chuan Liu

Ching-An Peng

Affiliations

Soon will be listed here.

Abstract

Reverse microemulsion was used as a template to fabricate chitosan-alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with the N/P ratios ranging from 5 to 20. These alginate-coated chitosan nanoparticles endocytosed by NIH 3T3 cells trigged swelling of transport vesicles which render gene escape before entering digestive endolysosomal compartment and concomitantly promote gene transfection rate. Results showed that DNA-encapsulated chitosan-alginate nanoparticles with average size of 64nm (N/P ratio of 5) could achieve the level of gene expression comparable with the one obtained by using polyethyleneimine-DNA complexes.

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