Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces Cerevisiae

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2007 Aug 19

PMID 17699586

Citations 141

Authors

Tomohiro Yorimitsu

Shadia Zaman

James R Broach

Daniel J Klionsky

Affiliations

Soon will be listed here.

Abstract

Autophagy is a highly conserved, degradative process in eukaryotic cells. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that the protein kinase A (PKA) and Sch9 signaling pathways regulate autophagy cooperatively in yeast. Autophagy is induced in cells when PKA and Sch9 are simultaneously inactivated. Mutant alleles of these kinases bearing a mutation that confers sensitivity to the ATP-analogue inhibitor C3-1'-naphthyl-methyl PP1 revealed that autophagy was induced independently of effects on Tor kinase. The PKA-Sch9-mediated autophagy depends on the autophagy-related 1 kinase complex, which is also essential for TORC1-regulated autophagy, the transcription factors Msn2/4, and the Rim15 kinase. The present results suggest that autophagy is controlled by the signals from at least three partly separate nutrient-sensing pathways that include PKA, Sch9, and TORC1.

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