Bipartite Stimulatory Action of the Hop2-Mnd1 Complex on the Rad51 Recombinase

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2007 Jul 20

PMID 17639080

Citations 82

Authors

Peter Chi

Joseph San Filippo

Michael G Sehorn

Galina V Petukhova

Patrick Sung

Affiliations

Soon will be listed here.

Abstract

The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We present evidence to implicate Hop2 as the major DNA-binding subunit and Mnd1 as the prominent Rad51 interaction entity. Hop2-Mnd1 stabilizes the Rad51-single-stranded DNA (ssDNA) nucleoprotein filament, the catalytic intermediate in recombination reactions. We also show that Hop2-Mnd1 enhances the ability of the Rad51-ssDNA nucleoprotein filament to capture duplex DNA, an obligatory step in the formation of the synaptic complex critical for DNA joint formation. Thus, our results unveil a bipartite mechanism of Hop2-Mnd1 in homologous DNA pairing: stabilization of the Rad51 presynaptic filament and duplex DNA capture to enhance synaptic complex formation.

Citing Articles

Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination.

Peng J, Chang H, Sun Y, Prentiss M, Li H, Chi P Nat Commun. 2024; 15(1):9266.

PMID: 39463417 PMC: 11514202. DOI: 10.1038/s41467-024-53641-3.

DSS1 restrains BRCA2's engagement with dsDNA for homologous recombination, replication fork protection, and R-loop homeostasis.

Huang Y, Li W, Foo T, Ji J, Wu B, Tomimatsu N Nat Commun. 2024; 15(1):7081.

PMID: 39152168 PMC: 11329725. DOI: 10.1038/s41467-024-51557-6.

TRF2-RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM-DNA2-dependent 5'-end resection.

Liang F, Rai R, Sodeinde T, Chang S Nucleic Acids Res. 2024; 52(16):9695-9709.

PMID: 39082275 PMC: 11381343. DOI: 10.1093/nar/gkae642.

Integrative Pan-Cancer Analysis Reveals the Oncogenic Role of MND1 and Validation of MND1's Role in Breast Cancer.

Zhang W, Xiao Y, Zhu X, Zhang Y, Xiang Q, Wu S J Inflamm Res. 2024; 17:4721-4746.

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The translocation activity of Rad54 reduces crossover outcomes during homologous recombination.

Sridalla K, Woodhouse M, Hu J, Scheer J, Ferlez B, Crickard J Nucleic Acids Res. 2024; 52(12):7031-7048.

PMID: 38828785 PMC: 11229335. DOI: 10.1093/nar/gkae474.

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