Heterodimeric Complexes of Hop2 and Mnd1 Function with Dmc1 to Promote Meiotic Homolog Juxtaposition and Strand Assimilation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Jul 14

PMID 15249670

Citations 60

Authors

Yi-Kai Chen

Chih-Hsiang Leng

Heidi Olivares

Ming-Hui Lee

Yuan-Chih Chang

Wen-Mei Kung

Shih-Chieh Ti

Yu-Hui Lo

Andrew H-J Wang

Chia-Seng Chang

Douglas K Bishop

Yi-Ping Hsueh

Ting-Fang Wang

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

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