A First Look at ARFome: Dual-coding Genes in Mammalian Genomes

Overview

Journal PLoS Comput Biol

Specialty Biology

Date 2007 May 22

PMID 17511511

Citations 42

Authors

Wen-Yu Chung

Samir Wadhawan

Radek Szklarczyk

Sergei Kosakovsky Pond

Anton Nekrutenko

Affiliations

Soon will be listed here.

Abstract

Coding of multiple proteins by overlapping reading frames is not a feature one would associate with eukaryotic genes. Indeed, codependency between codons of overlapping protein-coding regions imposes a unique set of evolutionary constraints, making it a costly arrangement. Yet in cases of tightly coexpressed interacting proteins, dual coding may be advantageous. Here we show that although dual coding is nearly impossible by chance, a number of human transcripts contain overlapping coding regions. Using newly developed statistical techniques, we identified 40 candidate genes with evolutionarily conserved overlapping coding regions. Because our approach is conservative, we expect mammals to possess more dual-coding genes. Our results emphasize that the skepticism surrounding eukaryotic dual coding is unwarranted: rather than being artifacts, overlapping reading frames are often hallmarks of fascinating biology.

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