Tilt Angle of Lipid Acyl Chains in Unilamellar Vesicles Determined by Ellipsometric Light Scattering

Overview

Journal Eur Phys J E Soft Matter

Publisher EDP Sciences

Specialty Biophysics

Date 2007 Apr 14

PMID 17431539

Citations 8

Authors

A Erbe

R Sigel

Affiliations

Soon will be listed here.

Abstract

Ellipsometric light scattering (ELS) at room temperature is applied to unilamellar vesicles (approximately 50 nm radius) of 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in the gel phase and of 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) in the liquid-crystaline phase. A high sensitivity of this technique to the local anisotropy is found. From the resulting local birefringence, a lower limit of (29 +/-0.5) degrees for the average tilt angle of the lipid chains of DPPC with respect to the membrane normal is estimated. This tilt angle value is slightly lower than literature values for the tilt angle in oriented lipid multi-bilayers on solid substrates.

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