A Riboswitch Regulates Expression of the Coenzyme B12-independent Methionine Synthase in Mycobacterium Tuberculosis: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Feb 20

PMID 17307844

Citations 46

Authors

Digby F Warner

Suzana Savvi

Valerie Mizrahi

Stephanie S Dawes

Affiliations

Soon will be listed here.

Abstract

We observed vitamin B(12)-mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B(12) sensitivity was mapped to a polymorphism in metH, encoding a coenzyme B(12)-dependent methionine synthase. Vitamin B(12)-resistant suppressor mutants of CDC1551 containing mutations in a B(12) riboswitch upstream of the metE gene, which encodes a B(12)-independent methionine synthase, were isolated. Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis.

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