Virus-specific T Cell Responses in Macaques Acutely Infected with SHIV(sf162p3)

Overview

Journal Virology

Specialty Microbiology

Date 2007 Feb 20

PMID 17307212

Citations 27

Authors

Bapi Pahar

Xiaolei Wang

Jason Dufour

Andrew A Lackner

Ronald S Veazey

Affiliations

Soon will be listed here.

Abstract

CD4(+) T helper and CD8(+) cytotoxic T lymphocyte responses are believed to play an important role in the control of primary HIV and SIV infection. However, the role of these cells in macaques acutely infected with SHIV(sf162p3) has not been well characterized. In this study, ten adult rhesus macaques were intravaginally infected with SHIV(sf162p3), and antigen-specific cytokine responses to SHIV-Tat, Nef, Gag and Env peptide pools were examined through 70 days post inoculation (p.i.) using ELISPOT and/or cytokine flow cytometry (CFC). Peak plasma viral replication occurred between 14 and 21 days p.i. followed by low to undetectable plasma viremia by 70 days of infection in most macaques. Although some animals had strong virus-specific cellular immune responses, many had weak or minimal responses that did not correlate with the post peak decline in plasma viremia.

Citing Articles

Immune perturbation following SHIV infection is greater in newborn macaques than in infants.

Shapiro M, Ordonez T, Pandey S, Mahyari E, Onwuzu K, Reed J JCI Insight. 2024; 9(19).

PMID: 39190496 PMC: 11466190. DOI: 10.1172/jci.insight.144448.

Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques.

Pahar B, Gray W, Fahlberg M, Grasperge B, Hunter M, Das A Viruses. 2022; 14(12).

PMID: 36560823 PMC: 9853323. DOI: 10.3390/v14122819.

Immune Responses and Viral Persistence in Simian/Human Immunodeficiency Virus SHIV.C.CH848-Infected Rhesus Macaques.

Ziani W, Bauer A, Lu H, Wang X, Wu X, Bar K J Virol. 2021; 95(9).

PMID: 33568508 PMC: 8104099. DOI: 10.1128/JVI.02198-20.

Quantification of Viral RNA and DNA Positive Cells in Tissues From Simian Immunodeficiency Virus/Simian Human Immunodeficiency Virus Infected Controller and Progressor Rhesus Macaques.

Pahar B, Kuebler D, Rasmussen T, Wang X, Srivastav S, Das A Front Microbiol. 2020; 10:2933.

PMID: 31921088 PMC: 6933296. DOI: 10.3389/fmicb.2019.02933.

Lack of T-cell-mediated IL-2 and TNFα production is linked to decreased CD58 expression in intestinal tissue during acute simian immunodeficiency virus infection.

Pan D, Das A, Srivastav S, Traina-Dorge V, Didier P, Pahar B J Gen Virol. 2018; 100(1):26-34.

PMID: 30480508 PMC: 6537620. DOI: 10.1099/jgv.0.001181.

References

Addo M, Yu X, Rathod A, Cohen D, Eldridge R, Strick D . Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1)-specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load. J Virol. 2003; 77(3):2081-92. PMC: 140965. DOI: 10.1128/jvi.77.3.2081-2092.2003. View

Veazey R, Ling B, Pandrea I, McClure H, Lackner A, Marx P . Decreased CCR5 expression on CD4+ T cells of SIV-infected sooty mangabeys. AIDS Res Hum Retroviruses. 2003; 19(3):227-33. DOI: 10.1089/088922203763315731. View

Cromwell M, Veazey R, Altman J, Mansfield K, Glickman R, Allen T . Induction of mucosal homing virus-specific CD8(+) T lymphocytes by attenuated simian immunodeficiency virus. J Virol. 2000; 74(18):8762-6. PMC: 116390. DOI: 10.1128/jvi.74.18.8762-8766.2000. View

Pahar B, Lackner A, Veazey R . Intestinal double-positive CD4+CD8+ T cells are highly activated memory cells with an increased capacity to produce cytokines. Eur J Immunol. 2006; 36(3):583-92. DOI: 10.1002/eji.200535520. View

Pitcher C, Hagen S, Walker J, Lum R, Mitchell B, Maino V . Development and homeostasis of T cell memory in rhesus macaque. J Immunol. 2001; 168(1):29-43. DOI: 10.4049/jimmunol.168.1.29. View

Schmitz J, Kuroda M, Santra S, Sasseville V, Simon M, Lifton M . Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. Science. 1999; 283(5403):857-60. DOI: 10.1126/science.283.5403.857. View

Monceaux V, Ho Tsong Fang R, Cumont M, Hurtrel B, Estaquier J . Distinct cycling CD4(+)- and CD8(+)-T-cell profiles during the asymptomatic phase of simian immunodeficiency virus SIVmac251 infection in rhesus macaques. J Virol. 2003; 77(18):10047-59. PMC: 224564. DOI: 10.1128/jvi.77.18.10047-10059.2003. View

Betts M, Krowka J, Kepler T, Davidian M, Christopherson C, Kwok S . Human immunodeficiency virus type 1-specific cytotoxic T lymphocyte activity is inversely correlated with HIV type 1 viral load in HIV type 1-infected long-term survivors. AIDS Res Hum Retroviruses. 1999; 15(13):1219-28. DOI: 10.1089/088922299310313. View

Kuroda M, Schmitz J, Charini W, Nickerson C, Lifton M, Lord C . Emergence of CTL coincides with clearance of virus during primary simian immunodeficiency virus infection in rhesus monkeys. J Immunol. 1999; 162(9):5127-33. View

10.

Harouse J, Gettie A, Tan R, Blanchard J, Cheng-Mayer C . Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs. Science. 1999; 284(5415):816-9. DOI: 10.1126/science.284.5415.816. View

11.

Pahar B, Li J, McChesney M . Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry. J Immunol Methods. 2005; 304(1-2):174-83. DOI: 10.1016/j.jim.2005.07.018. View

12.

Betts M, Nason M, West S, De Rosa S, Migueles S, Abraham J . HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood. 2006; 107(12):4781-9. PMC: 1895811. DOI: 10.1182/blood-2005-12-4818. View

13.

Veazey R, Shattock R, Pope M, Kirijan J, Jones J, Hu Q . Prevention of virus transmission to macaque monkeys by a vaginally applied monoclonal antibody to HIV-1 gp120. Nat Med. 2003; 9(3):343-6. DOI: 10.1038/nm833. View

14.

Mehandru S, Poles M, Tenner-Racz K, Horowitz A, Hurley A, Hogan C . Primary HIV-1 infection is associated with preferential depletion of CD4+ T lymphocytes from effector sites in the gastrointestinal tract. J Exp Med. 2004; 200(6):761-70. PMC: 2211967. DOI: 10.1084/jem.20041196. View

15.

Picker L, Hagen S, Lum R, Reed-Inderbitzin E, Daly L, Sylwester A . Insufficient production and tissue delivery of CD4+ memory T cells in rapidly progressive simian immunodeficiency virus infection. J Exp Med. 2004; 200(10):1299-314. PMC: 2211921. DOI: 10.1084/jem.20041049. View

16.

Waldrop S, Pitcher C, Peterson D, Maino V, Picker L . Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest. 1997; 99(7):1739-50. PMC: 507995. DOI: 10.1172/JCI119338. View

17.

Belyakov I, Kuznetsov V, Kelsall B, Klinman D, Moniuszko M, Lemon M . Impact of vaccine-induced mucosal high-avidity CD8+ CTLs in delay of AIDS viral dissemination from mucosa. Blood. 2005; 107(8):3258-64. PMC: 1895757. DOI: 10.1182/blood-2005-11-4374. View

18.

Borrow P, Lewicki H, Hahn B, Shaw G, Oldstone M . Virus-specific CD8+ cytotoxic T-lymphocyte activity associated with control of viremia in primary human immunodeficiency virus type 1 infection. J Virol. 1994; 68(9):6103-10. PMC: 237022. DOI: 10.1128/JVI.68.9.6103-6110.1994. View

19.

Van Rompay K, Singh R, Pahar B, Sodora D, Wingfield C, Lawson J . CD8+-cell-mediated suppression of virulent simian immunodeficiency virus during tenofovir treatment. J Virol. 2004; 78(10):5324-37. PMC: 400346. DOI: 10.1128/jvi.78.10.5324-5337.2004. View

20.

Reimann K, Tenner-Racz K, Racz P, Montefiori D, Yasutomi Y, Lin W . Immunopathogenic events in acute infection of rhesus monkeys with simian immunodeficiency virus of macaques. J Virol. 1994; 68(4):2362-70. PMC: 236713. DOI: 10.1128/JVI.68.4.2362-2370.1994. View