Contribution of Long Chain Fatty Acids to the Energy Supply of the Rat Kidney Cortex

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1975 Apr 9

PMID 172858

Citations 4

Authors

H Bertermann

G Gronow

A Schirmer

C Weiss

Affiliations

Soon will be listed here.

Abstract

Tubular fragments from rat kidney cortex were isolated by collagenase and suspended in an incubation medium containing a combination of several renal substrates. Substrate concentrations were in the physiological range. O2 uptake, total CO2 production, and the 14CO2 production from U-14C-labeled palmitate and oleate were measured. During the first minutes of incubation the CO2 production from palmitate and oleate was 10.5% or 6.3%, respectively, of the total CO3 produced. The RQ was 0.897. A subsequent decrease of the total CO2 production at a constant uptake of oxygen indicated a rising contribution of fatty acids to the fuel of respiration. The renal preference for substrates other than longchain fatty acids is discussed.

Citing Articles

The Structure of the Cardiac Mitochondria Respirasome Is Adapted for the β-Oxidation of Fatty Acids.

Panov A Int J Mol Sci. 2024; 25(4).

PMID: 38397087 PMC: 10889813. DOI: 10.3390/ijms25042410.

Long-Chain and Medium-Chain Fatty Acids in Energy Metabolism of Murine Kidney Mitochondria.

Panov A, Mayorov V, Dikalova A, Dikalov S Int J Mol Sci. 2023; 24(1).

PMID: 36613826 PMC: 9820327. DOI: 10.3390/ijms24010379.

Metabolic Syndrome and β-Oxidation of Long-Chain Fatty Acids in the Brain, Heart, and Kidney Mitochondria.

Panov A, Mayorov V, Dikalov S Int J Mol Sci. 2022; 23(7).

PMID: 35409406 PMC: 9000033. DOI: 10.3390/ijms23074047.

Targeted mitochondrial therapy using MitoQ shows equivalent renoprotection to angiotensin converting enzyme inhibition but no combined synergy in diabetes.

Ward M, Flemming N, Gallo L, Fotheringham A, McCarthy D, Zhuang A Sci Rep. 2017; 7(1):15190.

PMID: 29123192 PMC: 5680236. DOI: 10.1038/s41598-017-15589-x.

References

HOHENLEITNER F, SPITZER J . Changes in plasma free fatty acid concentrations on passage through the dog kidney. Am J Physiol. 1961; 200:1095-8. DOI: 10.1152/ajplegacy.1961.200.5.1095. View

Garland P, Newsholme E, Randle P . Regulation of glucose uptake by muscle. 9. Effects of fatty acids and ketone bodies, and of alloxan-diabetes and starvation, on pyruvate metabolism and on lactate-pyruvate and L-glycerol 3-phosphate-dihydroxyacetone phosphate concentration ratios in.... Biochem J. 1964; 93(3):665-78. PMC: 1214025. DOI: 10.1042/bj0930665. View

BURG M, ORLOFF J . Oxygen consumption and active transport in separated renal tubules. Am J Physiol. 1962; 203:327-30. DOI: 10.1152/ajplegacy.1962.203.2.327. View

DICKENS F, Simer F . The metabolism of normal and tumour tissue: The respiratory quotient, and the relationship of respiration to glycolysis. Biochem J. 1930; 24(5):1301-26. PMC: 1254669. DOI: 10.1042/bj0241301. View

IISALO E . Enzyme action on noradrenaline and adrenaline. Studies on bovine and guinea pig tissues in vitro with special reference to monoamine oxidase. Acta Pharmacol Toxicol (Copenh). 1962; 19(Suppl 1):1-90. View

Park H, King F, MacLeod M, PITTS R . Metabolism of lactate by the intact functioning kidney of the dog. Am J Physiol. 1973; 224(6):1463-7. DOI: 10.1152/ajplegacy.1973.224.6.1463. View

Balasse E . Inhibition of free fatty acid oxidation by acetoacetate in normal dogs. Eur J Clin Invest. 1970; 1(3):155-60. DOI: 10.1111/j.1365-2362.1970.tb00611.x. View

Grube G, Philipps K, Weiss C . [Effect of changes in the sodium concentration in the suspension medium and of strophanthin on the oxygen absorption of isolated rat kidney cells]. Z Gesamte Exp Med Einschl Exp Chir. 1967; 143(2):150-60. View

Weidemann M, KREBS H . The fuel of respiration of rat kidney cortex. Biochem J. 1969; 112(2):149-66. PMC: 1187688. DOI: 10.1042/bj1120149. View

10.

Barac-Nieto M, Cohen J . The metabolic fates of palmitate in the dog kidney in vivo. Evidence for incomplete oxidation. Nephron. 1971; 8(5):488-99. DOI: 10.1159/000179952. View

11.

KREBS H . The physiological role of the ketone bodies. Biochem J. 1961; 80:225-33. PMC: 1243987. DOI: 10.1042/bj0800225. View

12.

DIES F, Ramos G, Avelar E, Matos M . Relationship between renal substrate uptake and tubular sodium reabsorption in the dog. Am J Physiol. 1970; 218(2):411-6. DOI: 10.1152/ajplegacy.1970.218.2.411. View

13.

JOLIN T, Montes A . Daily rhythm of plasma glucose and insulin levels in rats. Horm Res. 1973; 4(3):153-6. DOI: 10.1159/000178303. View

14.

KREBS H . The regulation of the release of ketone bodies by the liver. Adv Enzyme Regul. 1966; 4:339-54. DOI: 10.1016/0065-2571(66)90027-6. View

15.

Aziz O . [Cross circulation experiments with unanesthetized rats. II. Endogenous secretion and plasma concentration of ADH]. Pflugers Arch. 1969; 311(4):355-72. DOI: 10.1007/BF00587230. View

16.

Barac-Nieto M, Cohen J . Nonesterified fatty acid uptake by dog kidney: effects of probenecid and chlorothiazide. Am J Physiol. 1968; 215(1):98-107. DOI: 10.1152/ajplegacy.1968.215.1.98. View

17.

Lee J, VANCE V, Cahill Jr G . Metabolism of C14-labeled substrates by rabbit kidney cortex and medulla. Am J Physiol. 1962; 203:27-36. DOI: 10.1152/ajplegacy.1962.203.1.27. View

18.

OCHWADT B, Schroder E, BETHGE H . [Studies on the metabolism of non-esterized fatty acids in an isolated dog kidney by means of C 14-palmitic acid]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965; 286(3):199-206. View

19.

Bassler K, Brinkrolf H . [The role of oxaloacetate in increased ketogenesis and in Antiketogenic action]. Z Gesamte Exp Med Einschl Exp Chir. 1971; 156(1):52-60. View

20.

NIETH H, Schollmeyer P . Substrate-utilization of the human kidney. Nature. 1966; 209(5029):1244-5. DOI: 10.1038/2091244a0. View