Effect of Culture Conditions on PLP and MAG Gene Expression in Rat Glioma C6 Cells

Overview

Journal Metab Brain Dis

Publisher Springer

Specialties Endocrinology
Neurology

Date 1991 Sep 1

PMID 1723486

Citations 2

Authors

M Kanoh

P Ye

W Zhu

R C Wiggins

G Konat

Affiliations

Soon will be listed here.

Abstract

The effects of culture conditions on the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) in rat glioma C6 cells was studied. Early passage (40-46) cells had higher steady-state level of PLP- and MAG-specific mRNA than late (100) passage cells when grown in defined (serum-free) medium. The PLP gene expression was increased whereas the MAG gene expression was reduced in the presence of 10% fetal calf serum in either passage. The level of both PLP- and MAG-specific messages was also directly related to the cell density indicating cell contact-induced stimulation of the gene expression. Furthermore, the cells apparently secrete factors into the medium, which upregulate the gene expression in autocrine fashion. The results also indicate a dissimilarity of regulatory mechanisms involved in the expression of the PLP and MAG genes.

Citing Articles

Ascorbic acid upregulates myelin gene expression in C6 glioma cells.

Laszkiewicz I, Wiggins R, Konat G Metab Brain Dis. 1992; 7(3):157-64.

PMID: 1279371 DOI: 10.1007/BF01000161.

Differential upregulation of PLP and MAG genes in C6 glioma cells by N2A neuroblastoma conditioned medium.

Kanoh M, Wiggins R, Konat G Metab Brain Dis. 1992; 7(3):147-56.

PMID: 1279370 DOI: 10.1007/BF01000160.

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