Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-density Microarray

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2007 Jan 19

PMID 17229859

Citations 84

Authors

Luciano Kleber de Souza Luna

Volker Heiser

Nicolas Regamey

Marcus Panning

Jan Felix Drexler

Sabue Mulangu

Leo Poon

Sigrid Baumgarte

Bert Jan Haijema

Laurent Kaiser

Christian Drosten

Affiliations

Soon will be listed here.

Abstract

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

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