A Novel Pancoronavirus RT-PCR Assay: Frequent Detection of Human Coronavirus NL63 in Children Hospitalized with Respiratory Tract Infections in Belgium

Overview

Journal BMC Infect Dis

Publisher Biomed Central

Specialty Infectious Diseases

Date 2005 Feb 3

PMID 15686594

Citations 105

Authors

Elien Moes

Leen Vijgen

Els Keyaerts

Kalina Zlateva

Sandra Li

Piet Maes

Krzysztof Pyrc

Ben Berkhout

Lia van der Hoek

Marc Van Ranst

Affiliations

Soon will be listed here.

Abstract

Background: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.

Methods: Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.

Results: Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.

Conclusions: Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.

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