Normalization of Boutique Two-color Microarrays with a High Proportion of Differentially Expressed Probes

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2007 Jan 6

PMID 17204140

Citations 35

Authors

Alicia Oshlack

Dianne Emslie

Lynn M Corcoran

Gordon K Smyth

Affiliations

Soon will be listed here.

Abstract

Normalization is critical for removing systematic variation from microarray data. For two-color microarray platforms, intensity-dependent lowess normalization is commonly used to correct relative gene expression values for biases. Here we outline a normalization method for use when the assumptions of lowess normalization fail. Specifically, this can occur when specialized boutique arrays are constructed that contain a subset of genes selected to test particular biological functions.

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