Gene Expression Profiles of Primary Colorectal Carcinomas, Liver Metastases, and Carcinomatoses

Overview

Journal Mol Cancer

Publisher Biomed Central

Specialties Molecular Biology
Oncology

Date 2007 Jan 5

PMID 17201907

Citations 41

Authors

Kristine Kleivi

Guro E Lind

Chieu B Diep

Gunn I Meling

Lin T Brandal

Jahn M Nesland

Ola Myklebost

Torleiv O Rognum

Karl-Erik Giercksky

Rolf I Skotheim

Ragnhild A Lothe

Affiliations

Soon will be listed here.

Abstract

Background: Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses by use of DNA microarrays. Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel.

Results: Transcriptome profiles of colorectal cancer metastases independent of tumor site, as well as separate profiles associated with primary carcinomas, liver metastases, or peritoneal carcinomatoses, were assessed by use of Bayesian statistics. Gains of chromosome arm 5p are common in peritoneal carcinomatoses and several candidate genes (including PTGER4, SKP2, and ZNF622) mapping to this region were overexpressed in the tumors. Expression signatures stratified on TP53 mutation status were identified across all tumors regardless of stage. Furthermore, the gene expression levels for the in vivo tumors were compared with an in vitro model consisting of cell lines representing all three tumor stages established from one patient.

Conclusion: By statistical analysis of gene expression data from primary colorectal carcinomas, liver metastases, and carcinomatoses, we are able to identify genetic patterns associated with the different stages of tumorigenesis.

Citing Articles

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Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases.

Gelli M, Desterke C, Bani M, Boige V, Ferte C, Dartigues P Cancers (Basel). 2023; 15(17).

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