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Validation of a Commercially Available Human Immunoturbidimetric Assay for Haptoglobin Determination in Canine Serum Samples

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Journal Vet Res Commun
Publisher Springer
Date 2006 Dec 21
PMID 17180450
Citations 6
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Abstract

Haptoglobin is a positive acute-phase protein with a valuable role as a marker of inflammation in both human and veterinary medicine. The aim of this study was to validate a commercially available immunoturbidimetric method designed for human haptoglobin determination (Izasa SA, Barcelona, Spain) for its use in canine samples. Cross-reactivity between anti-human haptoglobin antiserum and canine haptoglobin was found when agarose gel immunodiffusion and ELISA tests were performed. The use of canine pooled serum with haptoglobin concentration of 6.3 g/L as standard provided higher analytical range than commercially available standards. Intra-assay and inter-assay coefficients of variation were 2.49% and 4.60%, respectively. A linear regression model between immunoturbidimetric results and a previously validated spectrophotometric method (Tridelta Development Limited, Ireland) yielded a slope at 95% confidence interval of 0.94 (0.86, 1.02) and y-intercept at 95% confidence interval of 0.11 (-0.59, 0.82). No significant differences were produced by anticoagulants, lipaemia and bilirubinaemia, although haemolysis significantly decreased haptoglobin. A significant increase of haptoglobin concentration was detected in inflammatory conditions such as pyometra and leishmaniasis, in neoplastic conditions, and after glucocorticoid administration. Canine serum haptoglobin concentration can be reliably measured using the commercially available Izasa immunoturbidimetric method developed for human haptoglobin determination. This method is precise and accurate, provides a wider analytical range than previous reported methods, and can be easily automated and used for routine haptoglobin determination in canine samples.

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References
1.
Martinez-Subiela S, Tecles F, Eckersall P, Ceron J . Serum concentrations of acute phase proteins in dogs with leishmaniasis. Vet Rec. 2002; 150(8):241-4. DOI: 10.1136/vr.150.8.241. View

2.
Weidmeyer C, Solter P . Validation of human haptoglobin immunoturbidimetric assay for detection of haptoglobin in equine and canine serum and plasma. Vet Clin Pathol. 1996; 25(4):141-146. DOI: 10.1111/j.1939-165x.1996.tb00988.x. View

3.
KENT J, Goodall J . Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations. Equine Vet J. 1991; 23(1):59-66. DOI: 10.1111/j.2042-3306.1991.tb02716.x. View

4.
Martinez-Subiela S, Ginel P, Ceron J . Effects of different glucocorticoid treatments on serum acute phase proteins in dogs. Vet Rec. 2004; 154(26):814-7. DOI: 10.1136/vr.154.26.814. View

5.
Katnik I, Pupek M, Stefaniak T . Cross reactivities among some mammalian haptoglobins studied by a monoclonal antibody. Comp Biochem Physiol B Biochem Mol Biol. 1998; 119(2):335-40. DOI: 10.1016/s0305-0491(97)00358-1. View