Diagnostic Utility of Canine C-Reactive Protein, Haptoglobin, and 25-Hydroxyvitamin-D in Dogs with Nasal Cavity Disease

Overview

Journal Animals (Basel)

Date 2024 Oct 16

PMID 39409857

Authors

Sarah Rosch

Julia Woitas

Gerhard Ulrich Oechtering

Affiliations

Soon will be listed here.

Abstract

In this prospective blinded study, canine C-reactive protein (c-CRP), haptoglobin (HPT), and 25-hydroxyvitamin-D (25(OH)D) were investigated for their diagnostic value in 55 dogs with nasal cavity disease (ND). After comprehensive diagnostics including a culture-dependent microbiological examination (ME) of nasal swabs, 17 dogs were excluded due to additionally detected systemic diseases or steroid pre-treatment. Included were 25 dogs with malignant ND (13 carcinomas and 12 sarcomas) and 30 dogs with benign ND (7 benign tumors, 13 idiopathic rhinitis (IR), and 10 others), as well as 10 controls. In none of the 72 dogs with ND was primary bacterial rhinitis diagnosed. Although within the reference interval, compared to the controls, c-CRP was significantly higher in dogs with ND in general and in every subgroup except for benign tumors. Serum HPT concentrations were not different among groups. Compared to the controls, 25(OH)D concentrations were significantly lower ( = 0.041) in malignant ND and sarcomas ( = 0.025). Despite pre-treatment with antibiotics (40/54; 74.1%), in 23/51 (45%) dogs, the ME was positive. Cultivated bacteria did not differ significantly between nasal diseases. The serum markers were not significantly different regarding the positivity of ME. In conclusion, the investigated serum markers were not clinically useful for the reliable detection of canine ND, as was the ME. Because of the low number of dogs with IR and positive or negative ME, further studies regarding c-CRP are needed in a larger group of IR dogs without concomitant diseases to reliably evaluate its utility in IR dogs with suspected secondary bacterial nasal infection.

References

Eckersall P, Conner J, Harvie J . An immunoturbidimetric assay for canine C-reactive protein. Vet Res Commun. 1991; 15(1):17-24. DOI: 10.1007/BF00497786. View

Young L, Backus R . Serum 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D concentrations in adult dogs are more substantially increased by oral supplementation of 25-hydroxyvitamin D than by vitamin D. J Nutr Sci. 2017; 6:e30. PMC: 5672302. DOI: 10.1017/jns.2017.8. View

Louw J, Werbeck A, Louw M, Kotze T, Cooper R, Labadarios D . Blood vitamin concentrations during the acute-phase response. Crit Care Med. 1992; 20(7):934-41. DOI: 10.1097/00003246-199207000-00007. View

de La Puente-Yague M, Cuadrado-Cenzual M, Ciudad-Cabanas M, Hernandez-Cabria M, Collado-Yurrita L . Vitamin D: And its role in breast cancer. Kaohsiung J Med Sci. 2018; 34(8):423-427. DOI: 10.1016/j.kjms.2018.03.004. View

Hillstrom A, Hagman R, Tvedten H, Kjelgaard-Hansen M . Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein. Vet Clin Pathol. 2014; 43(2):235-43. PMC: 4257579. DOI: 10.1111/vcp.12150. View

Ceron J, Eckersall P, Martynez-Subiela S . Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol. 2005; 34(2):85-99. DOI: 10.1111/j.1939-165x.2005.tb00019.x. View

Pauly L, Junginger J, Oechtering G, Hewicker-Trautwein M, Rosch S . Expression of vascular endothelial growth factor receptor-2, epidermal growth factor receptor, cyclooxygenase-2, survivin, E-cadherin and Ki-67 in canine nasal carcinomas and sarcomas - a pilot study. Front Vet Sci. 2024; 11:1388493. PMC: 11391428. DOI: 10.3389/fvets.2024.1388493. View

Reid D, Toole B, Knox S, Talwar D, Harten J, OReilly D . The relation between acute changes in the systemic inflammatory response and plasma 25-hydroxyvitamin D concentrations after elective knee arthroplasty. Am J Clin Nutr. 2011; 93(5):1006-11. DOI: 10.3945/ajcn.110.008490. View

Windsor R, Johnson L, Sykes J, Drazenovich T, Leutenegger C, De Cock H . Molecular detection of microbes in nasal tissue of dogs with idiopathic lymphoplasmacytic rhinitis. J Vet Intern Med. 2006; 20(2):250-6. DOI: 10.1892/0891-6640(2006)20[250:mdomin]2.0.co;2. View

10.

CLAPPER W, MEADE G . NORMAL FLORA OF THE NOSE, THROAT, AND LOWER INTESTINE OF DOGS. J Bacteriol. 1963; 85:643-8. PMC: 278195. DOI: 10.1128/jb.85.3.643-648.1963. View

11.

Selting K, Sharp C, Ringold R, Thamm D, Backus R . Serum 25-hydroxyvitamin D concentrations in dogs - correlation with health and cancer risk. Vet Comp Oncol. 2014; 14(3):295-305. DOI: 10.1111/vco.12101. View

12.

San T, Bayar Muluk N, Cingi C . 1,25(OH)₂D₃ and specific IgE levels in children with recurrent tonsillitis, and allergic rhinitis. Int J Pediatr Otorhinolaryngol. 2013; 77(9):1506-11. DOI: 10.1016/j.ijporl.2013.06.019. View

13.

Young L, Backus R . Oral vitamin D supplementation at five times the recommended allowance marginally affects serum 25-hydroxyvitamin D concentrations in dogs. J Nutr Sci. 2016; 5:e31. PMC: 4976120. DOI: 10.1017/jns.2016.23. View

14.

Lowrie M, Penderis J, Eckersall P, Mclaughlin M, Mellor D, Anderson T . The role of acute phase proteins in diagnosis and management of steroid-responsive meningitis arteritis in dogs. Vet J. 2008; 182(1):125-30. DOI: 10.1016/j.tvjl.2008.05.001. View

15.

Vangrinsven E, Duprez J, Meex C, Taminiau B, Daube G, Billen F . Comparison of culture-dependent and -independent bacterial detection results on nasal swabs in dogs with nasal discharge. J Small Anim Pract. 2024; 65(6):376-386. DOI: 10.1111/jsap.13734. View

16.

Viitanen S, Laurila H, Lilja-Maula L, Melamies M, Rantala M, Rajamaki M . Serum C-reactive protein as a diagnostic biomarker in dogs with bacterial respiratory diseases. J Vet Intern Med. 2013; 28(1):84-91. PMC: 4895528. DOI: 10.1111/jvim.12262. View

17.

Rosch S, Woitas J, Neumann S, Alef M, Kiefer I, Oechtering G . Diagnostic benefits of platelet-to-lymphocyte, neutrophil-to-lymphocyte, and albumin-to-globulin ratios in dogs with nasal cavity diseases. BMC Vet Res. 2024; 20(1):44. PMC: 10837884. DOI: 10.1186/s12917-024-03876-5. View

18.

Lobetti R . Idiopathic lymphoplasmacytic rhinitis in 33 dogs. J S Afr Vet Assoc. 2015; 85(1):1151. DOI: 10.4102/jsava.v85i1.1151. View

19.

Tecles F, Martinez Subiela S, Petrucci G, Panizo C, Ceron J . Validation of a commercially available human immunoturbidimetric assay for haptoglobin determination in canine serum samples. Vet Res Commun. 2006; 31(1):23-36. DOI: 10.1007/s11259-006-3397-y. View

20.

Giammanco M, Di Majo D, La Guardia M, Aiello S, Crescimannno M, Flandina C . Vitamin D in cancer chemoprevention. Pharm Biol. 2015; 53(10):1399-434. DOI: 10.3109/13880209.2014.988274. View