Scaffolding As an Organizing Principle in Trans-translation. The Roles of Small Protein B and Ribosomal Protein S1

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2006 Dec 21

PMID 17179154

Citations 20

Authors

Reynald Gillet

Sukhjit Kaur

Wen Li

Marc Hallier

Brice Felden

Joachim Frank

Affiliations

Soon will be listed here.

Abstract

A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.

Citing Articles

Structural insights into the binding of bS1 to the ribosome.

DUrso G, Chat S, Gillet R, Giudice E Nucleic Acids Res. 2023; 51(7):3410-3419.

PMID: 36840711 PMC: 10123108. DOI: 10.1093/nar/gkad126.

Dynamic analysis of ribosome by a movie made from many three-dimensional electron-microscopy density maps.

Matsumoto A Biophys Physicobiol. 2019; 16:108-113.

PMID: 31131181 PMC: 6530885. DOI: 10.2142/biophysico.16.0_108.

Joachim Frank's Binding with the Ribosome.

Li W, Agrawal R Structure. 2019; 27(3):411-419.

PMID: 30595455 PMC: 11062599. DOI: 10.1016/j.str.2018.11.006.

Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA.

Kurita D, Miller M, Muto A, Buskirk A, Himeno H RNA. 2014; 20(11):1706-14.

PMID: 25246654 PMC: 4201823. DOI: 10.1261/rna.045773.114.

Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis.

Wower I, Zwieb C, Wower J BMC Mol Biol. 2014; 15:19.

PMID: 25220282 PMC: 4236655. DOI: 10.1186/1471-2199-15-19.

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