Accommodation of TmRNA-SmpB into Stalled Ribosomes: a Cryo-EM Study

Overview

Journal RNA

Specialty Molecular Biology

Date 2009 Dec 30

PMID 20038631

Citations 17

Authors

Felix Weis

Patrick Bron

Jean-Paul Rolland

Daniel Thomas

Brice Felden

Reynald Gillet

Affiliations

Soon will be listed here.

Abstract

In eubacteria, translation of defective messenger RNAs (mRNAs) produces truncated polypeptides that stall on the ribosome. A quality control mechanism referred to as trans-translation is performed by transfer-messenger RNA (tmRNA), a specialized RNA acting as both a tRNA and an mRNA, associated with small protein B (SmpB). So far, a clear view of the structural movements of both the protein and RNA necessary to perform accommodation is still lacking. By using a construct containing the tRNA-like domain as well as the extended helix H2 of tmRNA, we present a cryo-electron microscopy study of the process of accommodation. The structure suggests how tmRNA and SmpB move into the ribosome decoding site after the release of EF-Tu.GDP. While two SmpB molecules are bound per ribosome in a preaccommodated state, our results show that during accommodation the SmpB protein interacting with the small subunit decoding site stays in place while the one interacting with the large subunit moves away. Relative to canonical translation, an additional movement is observed due to the rotation of H2. This suggests that the larger movement required to resume translation on a tmRNA internal open reading frame starts during accommodation.

Citing Articles

Structures of tmRNA and SmpB as they transit through the ribosome.

Guyomar C, DUrso G, Chat S, Giudice E, Gillet R Nat Commun. 2021; 12(1):4909.

PMID: 34389707 PMC: 8363625. DOI: 10.1038/s41467-021-24881-4.

Ribosome Rescue Pathways in Bacteria.

Muller C, Crowe-McAuliffe C, Wilson D Front Microbiol. 2021; 12:652980.

PMID: 33815344 PMC: 8012679. DOI: 10.3389/fmicb.2021.652980.

RNA-protein distance patterns in ribosomes reveal the mechanism of translational attenuation.

Yu D, Zhang C, Qin P, Cornish P, Xu D Sci China Life Sci. 2014; 57(11):1131-9.

PMID: 25326828 PMC: 4365502. DOI: 10.1007/s11427-014-4753-8.

The bacterial translation stress response.

Starosta A, Lassak J, Jung K, Wilson D FEMS Microbiol Rev. 2014; 38(6):1172-201.

PMID: 25135187 PMC: 4227928. DOI: 10.1111/1574-6976.12083.

tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.

Himeno H, Kurita D, Muto A Front Genet. 2014; 5:66.

PMID: 24778639 PMC: 3985003. DOI: 10.3389/fgene.2014.00066.

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