Regulation of RKIP Binding to the N-region of the Raf-1 Kinase

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2006 Nov 14

PMID 17097642

Citations 18

Authors

Sungdae Park

Oliver Rath

Sandy Beach

Xiaoqin Xiang

Sharon M Kelly

Zhijun Luo

Walter Kolch

Kam C Yeung

Affiliations

Soon will be listed here.

Abstract

The Raf kinase inhibitory protein (RKIP) binds to Raf-1 interfering with binding of the MEK substrate and potentially also Raf-1 activation. In response to mitogen stimulation RKIP dissociates from Raf-1 and later re-associates. Here, using a combination of mutational approaches, biochemical studies, peptide arrays and plasmon surface resonance (BIAcore), we fine map and characterize a minimal 24 amino acid long RKIP binding domain in the Raf-1 N-region, which consists of constitutive elements at both flanks and a center element that is regulated by phosphorylation and enhances the re-binding of RKIP to Raf-1 in the later phase of mitogen stimulation.

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