The Vitronectin Receptor Serves As an Accessory Molecule for the Activation of a Subset of Gamma/delta T Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1991 Jan 1

PMID 1702138

Citations 29

Authors

K Roberts

W M Yokoyama

P J Kehn

E M Shevach

Affiliations

Soon will be listed here.

Abstract

Constitutive production of cytokines was observed in 3 of 12 gamma/delta T cell lines derived from murine epidermis and correlated with the expression of the C gamma 4, V delta 6 T cell receptor (TCR). After adaptation of one of the lines (T195/BW) to serum-free culture conditions, cessation of the "spontaneous" production of interleukin 4 (IL-4) was observed and IL-4 production could then by induced by the addition of RGD-containing extracellular matrix (ECM) proteins to the culture. The response to the ECM proteins could be completely inhibited by a mAb to the murine vitronectin receptor (VNR). However, the induction of IL-4 production could also be inhibited by anti-CD3 and by an anti-clonotypic mAb to the TCR-gamma/delta of T195/BW. As TCR-gamma/delta loss mutants of T195/BW also failed to respond to ECM proteins, these data demonstrate that engagement of the VNR by its ligand is necessary, but not sufficient, for the induction of IL-4 production. Furthermore, the VNR is expressed by many other T cell clones (both gamma/delta and alpha/beta), none of which produce lymphokines constitutively. Taken together, these observations strongly favor the view that not only is coexpression of the VNR and TCR required for the induction of IL-4 production, but that the TCR must also be engaged by its ligand, most likely a cell surface antigen expressed by the hybridoma itself.

Citing Articles

Recognition of synthetic polyanionic ligands underlies "spontaneous" reactivity of Vγ1 γδTCRs.

Dunst J, Glaros V, Englmaier L, Sandoz P, Onfelt B, Kisielow J J Leukoc Biol. 2020; 107(6):1033-1044.

PMID: 31943366 PMC: 7317387. DOI: 10.1002/JLB.2MA1219-392R.

Integral Roles for Integrins in γδ T Cell Function.

Siegers G Front Immunol. 2018; 9:521.

PMID: 29593745 PMC: 5859029. DOI: 10.3389/fimmu.2018.00521.

Dendritic cell expression of ADAM23 governs T cell proliferation and cytokine production through the α(v)β(3) integrin receptor.

Elizondo D, Andargie T, Marshall K, Zariwala A, Lipscomb M J Leukoc Biol. 2016; 100(5):855-864.

PMID: 27317750 PMC: 6608068. DOI: 10.1189/jlb.2HI1115-525R.

gammadeltaTCR ligands and lineage commitment.

Kreslavsky T, von Boehmer H Semin Immunol. 2010; 22(4):214-21.

PMID: 20447836 PMC: 2912151. DOI: 10.1016/j.smim.2010.04.001.

Crocidolite induces prostaglandin I(2) release mediated by vitronectin receptor and cyclooxygenase-2 in lung cells.

Leyva F, Roberts K Lung. 2010; 188(2):133-41.

PMID: 20155273 PMC: 2862624. DOI: 10.1007/s00408-010-9229-4.

References

Mosher D, Proctor R . Binding and factor XIIIa-mediated cross-linking of a 27-kilodalton fragment of fibronectin to Staphylococcus aureus. Science. 1980; 209(4459):927-9. DOI: 10.1126/science.7403857. View

Pommier C, Inada S, Fries L, Takahashi T, Frank M, Brown E . Plasma fibronectin enhances phagocytosis of opsonized particles by human peripheral blood monocytes. J Exp Med. 1983; 157(6):1844-54. PMC: 2187060. DOI: 10.1084/jem.157.6.1844. View

Kuusela P . Fibronectin binds to Staphylococcus aureus. Nature. 1978; 276(5689):718-20. DOI: 10.1038/276718a0. View

Vidovic D, Roglic M, McKune K, Guerder S, MacKay C, Dembic Z . Qa-1 restricted recognition of foreign antigen by a gamma delta T-cell hybridoma. Nature. 1989; 340(6235):646-50. DOI: 10.1038/340646a0. View

Holoshitz J, Koning F, Coligan J, de Bruyn J, Strober S . Isolation of CD4- CD8- mycobacteria-reactive T lymphocyte clones from rheumatoid arthritis synovial fluid. Nature. 1989; 339(6221):226-9. DOI: 10.1038/339226a0. View

McConnell T, Yokoyama W, Kikuchi G, Einhorn G, Stingl G, Shevach E . Delta-chains of dendritic epidermal T cell receptors are diverse but pair with gamma-chains in a restricted manner. J Immunol. 1989; 142(8):2924-31. View

Werb Z, Tremble P, Behrendtsen O, Crowley E, Damsky C . Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression. J Cell Biol. 1989; 109(2):877-89. PMC: 2115739. DOI: 10.1083/jcb.109.2.877. View

Hemler M, Huang C, Schwarz L . The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit. J Biol Chem. 1987; 262(7):3300-9. View

Nathan C, Srimal S, Farber C, Sanchez E, Kabbash L, Asch A . Cytokine-induced respiratory burst of human neutrophils: dependence on extracellular matrix proteins and CD11/CD18 integrins. J Cell Biol. 1989; 109(3):1341-9. PMC: 2115779. DOI: 10.1083/jcb.109.3.1341. View

10.

Stingl G, Gunter K, Tschachler E, Yamada H, Lechler R, Yokoyama W . Thy-1+ dendritic epidermal cells belong to the T-cell lineage. Proc Natl Acad Sci U S A. 1987; 84(8):2430-4. PMC: 304665. DOI: 10.1073/pnas.84.8.2430. View

11.

Yokoyama W, Koning F, Kehn P, Pereira G, Stingl G, Coligan J . Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation. J Immunol. 1988; 141(2):369-76. View

12.

Bluestone J, Cron R, Cotterman M, Houlden B, Matis L . Structure and specificity of T cell receptor gamma/delta on major histocompatibility complex antigen-specific CD3+, CD4-, CD8- T lymphocytes. J Exp Med. 1988; 168(5):1899-916. PMC: 2189096. DOI: 10.1084/jem.168.5.1899. View

13.

Hemler M, Glass D, Coblyn J, Jacobson J . Very late activation antigens on rheumatoid synovial fluid T lymphocytes. Association with stages of T cell activation. J Clin Invest. 1986; 78(3):696-702. PMC: 423654. DOI: 10.1172/JCI112629. View

14.

Hynes R . Integrins: a family of cell surface receptors. Cell. 1987; 48(4):549-54. DOI: 10.1016/0092-8674(87)90233-9. View

15.

Koning F, Yokoyama W, Maloy W, Stingl G, McConnell T, Cohen D . Expression of C gamma 4 T cell receptors and lack of isotype exclusion by dendritic epidermal T cell lines. J Immunol. 1988; 141(6):2057-62. View

16.

Horwitz A, Duggan K, Buck C, Beckerle M, Burridge K . Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage. Nature. 1986; 320(6062):531-3. DOI: 10.1038/320531a0. View

17.

Gunter K, Germain R, Kroczek R, Saito T, Yokoyama W, Chan C . Thy-1-mediated T-cell activation requires co-expression of CD3/Ti complex. Nature. 1987; 326(6112):505-7. DOI: 10.1038/326505a0. View

18.

Yokoyama W, Koning F, Stingl G, Bluestone J, Coligan J, Shevach E . Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer. J Exp Med. 1987; 165(6):1725-30. PMC: 2188374. DOI: 10.1084/jem.165.6.1725. View

19.

Porcelli S, Brenner M, Greenstein J, Balk S, Terhorst C, Bleicher P . Recognition of cluster of differentiation 1 antigens by human CD4-CD8-cytolytic T lymphocytes. Nature. 1989; 341(6241):447-50. DOI: 10.1038/341447a0. View

20.

Haregewoin A, Soman G, Hom R, Finberg R . Human gamma delta+ T cells respond to mycobacterial heat-shock protein. Nature. 1989; 340(6231):309-12. DOI: 10.1038/340309a0. View