Nucleotide Sequence and Expression in E. Coli of the Complete P4 Type VP4 from a G2 Serotype Human Rotavirus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1996 Jan 1

PMID 8634023

Citations 1

Authors

N P Mahajan

C D Rao

Affiliations

Soon will be listed here.

Abstract

The complete sequence of a P4 type VP4 gene from a G2 serotype human rotavirus, IS2, isolated in India has been determined. Although the IS2 VP4 is highly homologous to the other P4 type alleles, it contained acidic amino acid substitutions at several positions that make it acidic among the P4 type alleles that are basic. Moreover, comparative sequence analysis revealed unusual polymorphism in members of the P4 type at amino acid position 393 which is highly conserved in members of other VP4 types. To date, expression of complete VP4 in E. colic has not been achieved. In this study we present successful expression in E. coli of the complete VP4 as well as VP8* and VP5* cleavage subunits in soluble form as fusion proteins of the maltose-binding protein (MBP) and their purification by single-step affinity chromatography. The hemagglutinating activity exhibited by the recombinant protein was specifically inhibited by the antiserum raised against it. Availability of pure VP4 proteins should facilitate development of polyclonal and monoclonal antibodies (MAbs) for P serotyping of rotaviruses.

Citing Articles

Cloning, expression and sequence analysis of the classical swine fever virus nucleocapsid protein.

Liu J, Wong M, Chen P, Chang T Virus Genes. 1998; 16(2):225-34.

PMID: 9608668 DOI: 10.1023/a:1007976208935.

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