Stimulation of Dmc1-mediated DNA Strand Exchange by the Human Rad54B Protein

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2006 Sep 2

PMID 16945962

Citations 12

Authors

Naoyuki Sarai

Wataru Kagawa

Takashi Kinebuchi

Ako Kagawa

Kozo Tanaka

Kiyoshi Miyagawa

Shukuko Ikawa

Takehiko Shibata

Hitoshi Kurumizaka

Shigeyuki Yokoyama

Affiliations

Soon will be listed here.

Abstract

The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

Citing Articles

RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage Repair Homologous Recombination.

Selemenakis P, Sharma N, Uhrig M, Katz J, Kwon Y, Sung P Front Cell Dev Biol. 2022; 10:866601.

PMID: 35652094 PMC: 9149245. DOI: 10.3389/fcell.2022.866601.

BIME2, a novel gene required for interhomolog meiotic recombination in the protist model organism Tetrahymena.

Shodhan A, Novatchkova M, Loidl J Chromosome Res. 2017; 25(3-4):291-298.

PMID: 28803330 PMC: 5662671. DOI: 10.1007/s10577-017-9563-y.

The synthetic lethal killing of RAD54B-deficient colorectal cancer cells by PARP1 inhibition is enhanced with SOD1 inhibition.

McAndrew E, Lepage C, McManus K Oncotarget. 2016; 7(52):87417-87430.

PMID: 27902462 PMC: 5349998. DOI: 10.18632/oncotarget.13654.

Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing.

Morozumi Y, Ino R, Takaku M, Hosokawa M, Chuma S, Kurumizaka H Nucleic Acids Res. 2011; 40(7):3031-41.

PMID: 22156371 PMC: 3326331. DOI: 10.1093/nar/gkr1229.

Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination.

Ceballos S, Heyer W Biochim Biophys Acta. 2011; 1809(9):509-23.

PMID: 21704205 PMC: 3171615. DOI: 10.1016/j.bbagrm.2011.06.006.

References

Shinohara A, Ogawa H, Matsuda Y, Ushio N, Ikeo K, Ogawa T . Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet. 1993; 4(3):239-43. DOI: 10.1038/ng0793-239. View

Henricksen L, Umbricht C, Wold M . Recombinant replication protein A: expression, complex formation, and functional characterization. J Biol Chem. 1994; 269(15):11121-32. View

Van Komen S, Petukhova G, Sigurdsson S, Stratton S, Sung P . Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54. Mol Cell. 2000; 6(3):563-72. DOI: 10.1016/s1097-2765(00)00055-1. View

Solinger J, Lutz G, Sugiyama T, Kowalczykowski S, Heyer W . Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament. J Mol Biol. 2001; 307(5):1207-21. DOI: 10.1006/jmbi.2001.4555. View

Caldecott K . Mammalian DNA single-strand break repair: an X-ra(y)ted affair. Bioessays. 2001; 23(5):447-55. DOI: 10.1002/bies.1063. View

Whitaker S . DNA damage by drugs and radiation: what is important and how is it measured?. Eur J Cancer. 1992; 28(1):273-6. DOI: 10.1016/0959-8049(92)90432-2. View

Bishop D, Park D, Xu L, Kleckner N . DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell. 1992; 69(3):439-56. DOI: 10.1016/0092-8674(92)90446-j. View

Sung P . Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science. 1994; 265(5176):1241-3. DOI: 10.1126/science.8066464. View

Ward J . The complexity of DNA damage: relevance to biological consequences. Int J Radiat Biol. 1994; 66(5):427-32. DOI: 10.1080/09553009414551401. View

10.

Eisen J, Sweder K, HANAWALT P . Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions. Nucleic Acids Res. 1995; 23(14):2715-23. PMC: 307096. DOI: 10.1093/nar/23.14.2715. View

11.

HABU T, Taki T, West A, Nishimune Y, Morita T . The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res. 1996; 24(3):470-7. PMC: 145652. DOI: 10.1093/nar/24.3.470. View

12.

Kleckner N . Meiosis: how could it work?. Proc Natl Acad Sci U S A. 1996; 93(16):8167-74. PMC: 38641. DOI: 10.1073/pnas.93.16.8167. View

13.

Baumann P, Benson F, West S . Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell. 1996; 87(4):757-66. DOI: 10.1016/s0092-8674(00)81394-x. View

14.

Gupta R, Bazemore L, Golub E, Radding C . Activities of human recombination protein Rad51. Proc Natl Acad Sci U S A. 1997; 94(2):463-8. PMC: 19535. DOI: 10.1073/pnas.94.2.463. View

15.

Keeney S, Giroux C, Kleckner N . Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell. 1997; 88(3):375-84. DOI: 10.1016/s0092-8674(00)81876-0. View

16.

Essers J, Hendriks R, Swagemakers S, Troelstra C, de Wit J, BOOTSMA D . Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell. 1997; 89(2):195-204. DOI: 10.1016/s0092-8674(00)80199-3. View

17.

Li Z, Golub E, Gupta R, Radding C . Recombination activities of HsDmc1 protein, the meiotic human homolog of RecA protein. Proc Natl Acad Sci U S A. 1997; 94(21):11221-6. PMC: 23422. DOI: 10.1073/pnas.94.21.11221. View

18.

Petukhova G, Stratton S, Sung P . Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature. 1998; 393(6680):91-4. DOI: 10.1038/30037. View

19.

Swagemakers S, Essers J, de Wit J, Hoeijmakers J, Kanaar R . The human RAD54 recombinational DNA repair protein is a double-stranded DNA-dependent ATPase. J Biol Chem. 1998; 273(43):28292-7. DOI: 10.1074/jbc.273.43.28292. View

20.

Tan T, Essers J, Citterio E, Swagemakers S, de Wit J, Benson F . Mouse Rad54 affects DNA conformation and DNA-damage-induced Rad51 foci formation. Curr Biol. 1999; 9(6):325-8. DOI: 10.1016/s0960-9822(99)80142-0. View