A Self-splicing Group II Intron in the Mitochondrial Large Subunit RRNA (LSUrRNA) Gene of the Eukaryotic Alga Scenedesmus Obliquus

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 May 11

PMID 1692614

Citations 21

Authors

U Kuck

I Godehardt

U Schmidt

Affiliations

Soon will be listed here.

Abstract

The DNA sequence has been determined of the 3' terminus from the mitochondrial large subunit ribosomal RNA (LSUrRNA) gene of the eukaryotic green alga Scenedesmus obliquus (strain KS3/2). The gene contains two intervening sequences with characteristic sequence motifs of group II and group I introns respectively. The exon/intron boundaries of the introns have been revealed by sequence determination of the mature rRNA. During RNA processing of the precursor RNA, several abundant RNA molecules are stably maintained in addition to the mature rRNA in vivo. In vitro transcripts of the LSUrRNA gene containing the group II intron (608 bp) display a strong 'self-splicing' activity under high salt conditions. The 608 bp intron is the first group II intron reported to be integrated into a LSUrRNA gene and represents the smallest self-splicing group II intron from eukaryotic organelles so far described.

Citing Articles

Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease.

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Function of chloroplast RNA-binding proteins.

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The complete mitochondrial DNA sequence of Scenedesmus obliquus reflects an intermediate stage in the evolution of the green algal mitochondrial genome.

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The reverse-transcriptase-like proteins encoded by group II introns in the mitochondrial genome of the brown alga Pylaiella littoralis belong to two different lineages which apparently coevolved with the group II ribosyme lineages.

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