Evidence That More Than 90% of Beta-Glucuronidase-Expressing Cells After Particle Bombardment Directly Receive the Foreign Gene in Their Nucleus

Overview

Journal Plant Physiol

Specialty Physiology

Date 1991 Oct 1

PMID 16668476

Citations 11

Authors

T Yamashita

A Iida

H Morikawa

Affiliations

Soon will be listed here.

Abstract

Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.

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References

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