Efficiency of Particle-bombardment-mediated Transformation is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco

Overview

Journal Plant Physiol

Specialty Physiology

Date 1991 Dec 1

PMID 16668589

Citations 10

Authors

A Iida

T Yamashita

Y Yamada

H Morikawa

Affiliations

Soon will be listed here.

Abstract

Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.

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References

Yamashita T, Iida A, Morikawa H . Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus. Plant Physiol. 1991; 97(2):829-31. PMC: 1081084. DOI: 10.1104/pp.97.2.829. View