Transient Expression of the Beta-glucuronidase Gene in Tissues of Arabidopsis Thaliana by Bombardment-mediated Transformation
Overview
Molecular Biology
Authors
Affiliations
Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.
Suitable transfection methods for single particle tracing in plant suspension cells.
Gohring J, Fulcher N, Schilcher K, Barta A, Jacak J Plant Methods. 2014; 10:15.
PMID: 24991230 PMC: 4076440. DOI: 10.1186/1746-4811-10-15.
Lu Y, Chen X, Wu Y, Wang Y, He Y, Wu Y PLoS One. 2013; 8(2):e57171.
PMID: 23468926 PMC: 3582559. DOI: 10.1371/journal.pone.0057171.
Orel N, Puchta H Plant Mol Biol. 2003; 51(4):523-31.
PMID: 12650618 DOI: 10.1023/a:1022324205661.