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Transient Expression of the Beta-glucuronidase Gene in Tissues of Arabidopsis Thaliana by Bombardment-mediated Transformation

Overview
Journal Mol Biotechnol
Publisher Springer
Date 1999 Sep 30
PMID 10503241
Citations 3
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Abstract

Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.

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References
1.
Gamborg O, Miller R, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968; 50(1):151-8. DOI: 10.1016/0014-4827(68)90403-5. View

2.
Ye G, Daniell H, Sanford J . Optimization of delivery of foreign DNA into higher-plant chloroplasts. Plant Mol Biol. 1990; 15(6):809-19. DOI: 10.1007/BF00039421. View

3.
Ingelbrecht I, Herman L, Dekeyser R, Van Montagu M, Depicker A . Different 3' end regions strongly influence the level of gene expression in plant cells. Plant Cell. 1989; 1(7):671-80. PMC: 159803. DOI: 10.1105/tpc.1.7.671. View

4.
Finer J, Vain P, Jones M, McMullen M . Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Rep. 2013; 11(7):323-8. DOI: 10.1007/BF00233358. View

5.
Bruce W, Christensen A, Klein T, Fromm M, Quail P . Photoregulation of a phytochrome gene promoter from oat transferred into rice by particle bombardment. Proc Natl Acad Sci U S A. 1989; 86(24):9692-6. PMC: 298567. DOI: 10.1073/pnas.86.24.9692. View