Purification and Characterization of Abundant Secreted Protein in Suspension-cultured Pumpkin Cells : Abundant Secreted Protein May Be a Chitinase

Overview

Journal Plant Physiol

Specialty Physiology

Date 1990 Jul 1

PMID 16667554

Citations 4

Authors

M Esaka

K Enoki

B Kouchi

T Sasaki

Affiliations

Soon will be listed here.

Abstract

The abundant secreted protein with molecular weight of 32,000 was purified from the culture medium of suspension-cultured pumpkin (Cucurbita sp.) cells. Two steps, ammonium sulfate fractionation and Sepharose 6B column chromatography, were sufficient for purification to homogeneity. Antibodies against the pure protein were used to show that a protein of the same size is made by callus cells. There is considerable homology between the amino-terminal amino acid sequence of this secreted protein and chitinase isolated from tobacco (Nicotiana tabacum L.) or bean (Phaseolus vulgaris L.).

Citing Articles

Nucellar callus of 'Femminello' lemon, selected for tolerance toPhoma tracheiphila toxin, shows enhanced release of chitinase and glucanase into the culture medium.

Gentile A, Tribulato E, Deng Z, Galun E, Fluhr R, Vardi A Theor Appl Genet. 2013; 86(5):527-32.

PMID: 24193699 DOI: 10.1007/BF00838705.

Root secretion of defense-related proteins is development-dependent and correlated with flowering time.

De-la-Pena C, Badri D, Lei Z, Watson B, Brandao M, Silva-Filho M J Biol Chem. 2010; 285(40):30654-65.

PMID: 20682788 PMC: 2945560. DOI: 10.1074/jbc.M110.119040.

Specific and abundant secretion of a novel hydroxyproline-rich glycoprotein from salt-adapted winged bean cells.

Esaka M, Hayakawa H, Hashimoto M, Matsubara N Plant Physiol. 1992; 100(3):1339-45.

PMID: 16653126 PMC: 1075787. DOI: 10.1104/pp.100.3.1339.

What's new in chitinase research?.

Flach J, Pilet P, Jolles P Experientia. 1992; 48(8):701-16.

PMID: 1516675 DOI: 10.1007/BF02124285.

References

Esaka M, Uchida M, Fukui H, Kubota K, Suzuki K . Marked increase in ascorbate oxidase protein in pumpkin callus by adding copper. Plant Physiol. 1988; 88(3):656-60. PMC: 1055640. DOI: 10.1104/pp.88.3.656. View

Camacho Henriquez A, Sanger H . Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the "pathogenesis-related" protein p 14. Arch Virol. 1982; 74(2-3):181-96. DOI: 10.1007/BF01314711. View

Boller T, Vogeli U . Vacuolar localization of ethylene-induced chitinase in bean leaves. Plant Physiol. 1984; 74(2):442-4. PMC: 1066699. DOI: 10.1104/pp.74.2.442. View

Legrand M, Kauffmann S, Geoffroy P, Fritig B . Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases. Proc Natl Acad Sci U S A. 1987; 84(19):6750-4. PMC: 299162. DOI: 10.1073/pnas.84.19.6750. View

Metraux J, Burkhart W, Moyer M, Dincher S, Middlesteadt W, Williams S . Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional lysozyme/chitinase. Proc Natl Acad Sci U S A. 1989; 86(3):896-900. PMC: 286585. DOI: 10.1073/pnas.86.3.896. View

Chrispeels M, Varner J . Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967; 42(3):398-406. PMC: 1086548. DOI: 10.1104/pp.42.3.398. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Shinshi H, Mohnen D, Meins F . Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987; 84(1):89-93. PMC: 304147. DOI: 10.1073/pnas.84.1.89. View

Hedrick S, Bell J, Boller T, Lamb C . Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection. Plant Physiol. 1988; 86(1):182-6. PMC: 1054451. DOI: 10.1104/pp.86.1.182. View

10.

Kauffmann S, Legrand M, Geoffroy P, Fritig B . Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity. EMBO J. 1987; 6(11):3209-12. PMC: 553771. DOI: 10.1002/j.1460-2075.1987.tb02637.x. View

11.

Kombrink E, Schroder M, Hahlbrock K . Several "pathogenesis-related" proteins in potato are 1,3-beta-glucanases and chitinases. Proc Natl Acad Sci U S A. 1988; 85(3):782-6. PMC: 279639. DOI: 10.1073/pnas.85.3.782. View

12.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

13.

van Loon L, van Kammen A . Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN". II. Changes in protein constitution after infection with tobacco mosaic virus. Virology. 1970; 40(2):190-211. DOI: 10.1016/0042-6822(70)90395-8. View